绵羊肺炎支原体人工感染SPF鸡胚的研究  

Experimental Infection of Mycoplasma ovipneumoniae in SPF Chicken Embryos

作　　者：袁婷 郝华芳[2] 马丽娜[2] 陈胜利[2] 颜新敏[2] 吴娅琴 蔡亚婷 孙延鸣[1] 储岳峰[2] YUAN Ting;HAO Huafang;MA Lina;CHEN Shengli;YAN Xinmin;WU Yaqin;CAI Yating;SUN Yanming;CHU Yuefeng(College of Animal Science and Technology,Shihezi University,Shihezi 832001,China;State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)

机构地区：[1]石河子大学动物科技学院,石河子832001 [2]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,兰州730046

出　　处：《中国畜牧兽医》2020年第9期2988-2996,共9页China Animal Husbandry & Veterinary Medicine

基　　金：国家重点研发计划项目子课题(2017YFD0500905);家畜疫病病原生物学国家重点实验室重大成果培育项目(2016-7)。

摘　　要：为探索SPF鸡胚作为绵羊肺炎支原体实验室感染模型的可行性,本试验用不同浓度绵羊肺炎支原体(10^8、10^9、10^10 ccu/mL)经由卵黄囊和尿囊腔两个部位接种7日龄SPF鸡胚,通过统计鸡胚死亡情况和不同鸡胚组织样品中绵羊肺炎支原体检测阳性率(PCR检测和支原体分离鉴定),确定绵羊肺炎支原体鸡胚感染方式、感染剂量和最佳分离部位,再用3株不同来源的绵羊肺炎支原体分离株感染鸡胚,观察其对鸡胚的致病力。结果表明,绵羊肺炎支原体感染鸡胚最佳接种途径为卵黄囊接种,感染剂量为10^9 ccu/mL、0.2 mL/只,最佳分离部位为卵黄液。3株支原体均能感染和致死鸡胚,并均能从卵黄液中分离到绵羊肺炎支原体,但对鸡胚的致病性存在一定差异:FL3株致鸡胚死亡率为45%,略高于MoGH3-3株(40%),二者均高于A3株(25%),但差异均不显著(P>0.05);FL3株鸡胚检测阳率为100%,高于MoGH3-3株(85%)及A3株(90%),但差异也不显著(P>0.05)。本试验初步确定了绵羊肺炎支原体可感染和致死SPF鸡胚,不同分离株对SPF鸡胚致病力有差异,表明SPF鸡胚可作为下一步建立绵羊肺炎支原体实验室感染模型的候选,为绵羊肺炎支原体致病性研究和疫苗研制奠定基础。In order to evaluate the SPF chicken embryos as a candidate of the experimental model of Mycoplasma ovipneumoniae(Mo)infection,the 7-day-old SPF chicken embryos were infected with different concentrations of Mo(10^8,10^9 and 10^10 ccu/mL)by injection of vitelline fluid and allantoic cavity.The mortality and Mo detection rate(PCR detection and isolation)of infected chicken embryos were employed to evaluate the optimal inoculation route,dose and sampling site.Three different isolates of Mo were submitted to injection of chicken embryos for pathogenicity comparison.The results showed that the optimal inoculation route and dose were vitelline fluid injection with 0.2 mL(10^9 ccu/mL)Mo per embryo and the sampling site for detection was also vitelline fluid.Three Mo isolates revealed good infectivity and lethality to the chicken embryo.The chicken embryos mortality caused by FL3 strain(45%)and MoGH3-3(40%)were both higher than that by A3 strain(25%),but with no significance(P>0.05).The detection rate of Mo from the chicken embryos in FL3 group(100%)was higher than that of MoGH3-3 strain(85%)and that of A3 strain(90%),but with no significance(P>0.05).This experiment preliminarily proved that the SPF chicken embryos could be infected and partially killed by Mo,and the virulence of different strains of Mo to the chicken embryos was different.The results provided the data for further establishment of the chicken embryo infection model of Mo,which would benefit the research on Mo pathogenicity and vaccine development.

关 键 词：绵羊肺炎支原体 感染 SPF鸡胚 

分 类 号：S858.26[农业科学—临床兽医学]