严重急性呼吸综合征冠状病毒2型4种结构蛋白特性分析  

Characteristics of Four Kinds of Structural Proteins of SARS-CoV-2

作　　者：李雪[1] 任林柱[1] Li Xue;Ren Linzhu(College of Animal Sciences,Jilin University,Changchun,Jilin 130062,China)

机构地区：[1]吉林大学动物科学学院,吉林长春130062

出　　处：《中国动物检疫》2020年第10期92-98,共7页China Animal Health Inspection

基　　金：国家重点研发计划项目(2017YFD0500103);国家自然科学基金面上项目(31772747);吉林省教育厅十三五科学技术项目(JJKH20190172KJ)。

摘　　要：为深入了解严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)S、M、E、N蛋白的分子生物学特征和基本结构特征,选取S、M、E、N蛋白序列进行生物信息学分析和预测,使用DNAstar、SignalP、TMHHM、PSORT Prediction、BUSCA和ABCpred软件,分析并相互验证4种蛋白的结构域特征和S蛋白的潜在B细胞抗原表位,结果预测出了4种结构蛋白的功能结构域,并分析预测出S蛋白潜在的B细胞抗原表位为25~29、75~81、112~116、148~152、773~779 aa。研究结果可为SARS-CoV-2相关的分子生物学和结构生物学研究提供参考,并为疫苗开发等提供理论依据。In order to further identify the molecular biological characteristics and general structural characteristics of S,M,E and N proteins of severe acute respiratory syndrome coronavirus type 2(SARS-CoV-2),the amino acid sequences of the above proteins were selected for bioinformatics analysis and prediction.The structural domain characteristics of the above proteins and the potential B cell epitopes of S protein were analyzed and mutually verified by DNAstar,SignalP,TMHHM,PSORT Prediction,BUSCA and ABCpred softwares.The results showed that the functional domains of the four proteins were predicted,and the potential B cell epitopes of S protein was analyzed and predicted to be 25 to 29,75 to 81,112 to 116,148 to152 and 773 to 779 aa.Based on the results,some references were provided for further study on molecular biology and structural biology related to SARS-CoV-2,and theoretical basis was provided for vaccine development.

关 键 词：严重急性呼吸综合征冠状病毒2型(SARS-CoV-2) 结构蛋白 结构域特征 抗原表位 生物信息学分析 分子生物学 

分 类 号：S852.65[农业科学—基础兽医学]