良姜胃疡胶囊高效液相色谱特征指纹图谱研究  

HPLC characteristic chromatographic profiles of Liangjiang Weiyang capsules

作　　者：徐国波 雷丹丹[1,2] 肖耀华 陈广桂 何迅 廖尚高 XU Guo-bo;LEI Dan-dan;XIAO Yao-hua;CHEN Guang-gui;HE Xun;LIAO Shang-gao(School of Pharmacy,Guizhou Medical University,Gui'an New District Guizhou 550025;National Engineering Research Center for Miao Medicine&Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine,Ministry of Education,Guiyang 550004)

机构地区：[1]贵州医科大学药学院,贵州贵安新区550025 [2]国家苗药工程技术中心及民族药与中药开发应用教育部工程研究中心,贵阳550004

出　　处：《中南药学》2020年第9期1503-1507,共5页Central South Pharmacy

基　　金：贵州省科技计划项目(No.黔科合支撑[2017]2888);贵州省高层次创新型人才百层次人才(No.黔科合平台人才[2020]6011);贵州省科技创新人才团队(No.黔科合平台人才[2020]5006)。

摘　　要：目的建立良姜胃疡胶囊的HPLC指纹图谱,为该制剂的质量控制提供参考。方法采用Diamonsil C18色谱柱(150 mm×4.6 mm,5μm)进行检测,以甲醇-0.1%甲酸水为流动相,梯度洗脱,流速1.0 mL·min^-1,检测波长334 nm,柱温30℃,进样量10μL。运用“中药色谱指纹图谱相似度评价系统”(2012版)对11批良姜胃疡胶囊化学成分指纹图谱进行相似度计算,通过对照品比较指认图谱的色谱峰,通过良姜胃疡胶囊组方单味药和制剂的HPLC比较,分析制剂中化学成分的归属。结果建立了11批良姜胃疡胶囊指纹图谱共有模式,标定了24个共有色谱峰,并指认了其中7个色谱峰;11批良姜胃疡胶囊与对照指纹图谱的相似度均>0.90。结论该方法具有良好的精密度、重复性和稳定性,建立的指纹图谱可为良姜胃疡胶囊的质量评价提供参考。Objective To establish the HPLC fingerprints of Liangjiang Weiyang capsules to provide a reference for their quality control.Methods Diamonsil C18 column(150 mm×4.6 mm,5μm)was used with a mobile phase consisted of methanol-0.1%formic acid in a gradient mode.The flow rate was 1.0 mL·min^-1,the detection wavelength was 334 nm,the column temperature was 30℃,and the injection volume was 10μL.The chemical fingerprint similarity of 11 batches of Liangjiang Weiyang capsules was calculated with the“Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine”(2012)by the Chinese Pharmacopoeia Commission.The main peaks in the fingerprints were identified by comparing the retention time of the reference substances.The source and attribution of chemical components in this preparation were analyzed by comparing with those of the single herb.Results Fingerprints of 11 batches of Liangjiang Weiyang capsules were established.Totally 24 common peaks were established in different batches of Liangjiang Weiyang capsules and 7 peaks were identified.Compared with the reference fingerprints,the similarities of 11 batches of Liangjiang Weiyang capsules were over 0.90.Conclusion This method is accurate,stable and reproducible,which provides a reference for the identification and quality evaluation of Liangjiang Weiyang capsules.

关 键 词：良姜胃疡胶囊 指纹图谱 高效液相色谱 质量控制 相似度 

分 类 号：R284[医药卫生—中药学] O657.72[医药卫生—中医学]