妊娠期高血压疾病患者母儿血清及胎盘组织热休克蛋白27的表达及临床意义  

Expression and clinical significance of heat shock protein 27 in maternal and fetal serum and placenta of patients with hypertensive disorder complicating pregnancy

作　　者：李认认 朱锦明[2] 王凤珍 LI Renren;ZHU Jinming;WANG Fengzhen(Graduate School,Xuzhou Medical University,Jiangsu Province,Xuzhou221004,China;Department of Obstetrics,Xuzhou Maternal and Child Health Care Hospital,Jiangsu Province,Xuzhou221009,China;Department of Pharmacy,Affiliated Hospital of Xuzhou Medical University,Jiangsu Province,Xuzhou221006,China)

机构地区：[1]徐州医科大学研究生学院,江苏徐州221004 [2]江苏省徐州市妇幼保健院产科,江苏徐州221009 [3]徐州医科大学附属医院药学部,江苏徐州221006

出　　处：《中国医药导报》2020年第27期104-107,共4页China Medical Herald

基　　金：国家自然科学基金青年科学基金项目(81803473)。

摘　　要：目的研究热休克蛋白27(HSP27)在妊娠期高血压疾病孕妇静脉血、脐血及胎盘组织中的表达及临床意义。方法选取2018年6月—2019年6月于江苏省徐州市妇幼保健院分娩的妊娠期高血压疾病孕妇90例[其中妊娠期高血压组、子痫前期(非重度)组和重度子痫前期组,各30例],同期另收集健康孕妇30例作为对照组。采用酶联免疫吸附法检测孕妇静脉血和脐血中HSP27水平;采用RT-PCR、免疫组织化学法检测胎盘组织HSP27 mRNA和蛋白的表达量。结果对照组、妊娠期高血压组、子痫前期(非重度)组、重度子痫前期组孕妇静脉血、脐血中HSP27水平均呈递增趋势,各组之间差异有高度统计学意义(P<0.01)。对照组、妊娠期高血压组、子痫前期(非重度)组、重度子痫前期组胎盘组织HSP27 mRNA表达呈递增趋势,各组之间差异有统计学意义(P<0.05)。对照组、妊娠期高血压组、子痫前期(非重度)组、重度子痫前期组胎盘组织HSP27蛋白表达量呈递增趋势,各组之间差异有统计学意义(P<0.05)。结论妊娠期高血压疾病孕妇静脉血、脐血和胎盘组织中HSP27呈高表达,且HSP27的表达与妊娠期高血压疾病严重程度有关,提示HSP27可能参与妊娠期高血压疾病的发生、发展。Objective To investigate the expression and clinical significance of heat shock protein 27(HSP27)in maternal and fetal serum and placental of patients with hypertensive disorders of pregnancy(HDP).Methods Ninety pregnant women with gestational hypertension delivered in Xuzhou Maternal and Child Health Hospital in Jiangsu Province from June 2018 to June 2019 were selected(including 30 cases of gestational hypertension group,30 cases of non severe preeclampsia group and 30 cases of severe preeclampsia group).Another 30 healthy pregnant women were selected as control group.The levels of HSP27 in venous blood and cord blood of pregnant women were detected by enzyme linked immunosorbent assay,and the expressions of HSP27 mRNA and protein in placenta were detected by RT-PCR and immunohistochemistry.Results The levels of HSP27 in venous blood and cord blood of pregnant women in control group,gestational hypertension group,preeclampsia(non severe)group and severe preeclampsia group showed an increasing trend,and the differences among all groups were highly statistically significant(P<0.01).The expression of HSP27 mRNA in placental tissues of control group,pregnancy induced hypertension group,preeclampsia(non severe)group and severe preeclampsia group showed an increasing trend,and the differences among all groups were statistically significant(P<0.05).The expression of HSP27 protein in placental tissues of control group,pregnancy induced hypertension group,preeclampsia(non severe)group and severe preeclampsia group showed an increasing trend,and the differences among all groups were statistically significant(P<0.05).Conclusion HSP27 is highly expressed in venous blood,umbilical blood and placental of pregnant women with HDP,and the expression of HSP27 is correlated with the severity of HDP,suggesting that HSP27 may play a role in the development of HDP.

关 键 词：妊娠期高血压疾病 热休克蛋白27 胎盘 脐血 母血 

分 类 号：R714.246[医药卫生—妇产科学]