常压高浓度吸氧对大鼠缺血再灌注肾损伤的影响  

Effect of normobaric hyperoxia on renal injury induced by ischemia-reperfusion in rats

作　　者：裴军 宋赏 余丹丹 杨超 徐元高[1] 李凯[3] 罗光恒 王元林[3] 孙发 石华[3] 冯梅[4] 徐述雄[3] Pei Jun;Song Shang;Yu Dandan;Yang Chao;Xu Yuangao;Li Kai;Luo Guangheng;Wang Yuanlin;Sun Fa;Shi Hua;Feng Mei;Xu Shuxiong(Department of Urology,People's Hospital Affiliated to Guizhou Medical University,Guiyang 550002,China;Graduate School,Zunyi Medical University,Zunyi 563003,China;Department of Urology,Guizhou Provincial People's Hospital,Guiyang 550002,China;Department of Hyperbaric Oxygen,Guizhou Provincial People's Hospital,Guiyang 550002,China)

机构地区：[1]贵州医科大学附属人民医院泌尿外科,贵阳550002 [2]遵义医科大学研究生院,563003 [3]贵州省人民医院泌尿外科,贵阳550002 [4]贵州省人民医院高压氧科,贵阳550002

出　　处：《中华实验外科杂志》2020年第7期1272-1276,共5页Chinese Journal of Experimental Surgery

基　　金：贵州省科技计划项目(黔科合基础2016-1090、黔科合LH-2016-7149);贵阳市科技计划项目(筑科合同20161001-30);贵州省高层次创新型人才培养(GZSYQCC-2014-005)。

摘　　要：目的探讨常压高浓度吸氧对大鼠缺血再灌注肾损伤的作用及其机制。方法2019年6月至2019年8月,选取成年雄性SD大鼠(购自辽宁长生生物技术股份有限公司)18只,均予切除左侧肾脏建立孤肾模型,1周后数字随机分配到3个组中,每组6只,分别为假手术组(S组)、对照组(C组)和实验组(E组)。S组仅钝性分离右肾肾蒂,但不予缺血处理;C组和E组均以动脉夹夹闭肾蒂致右肾缺血40 min。24 h后3组均置于密闭氧舱中,S组和C组吸入常规浓度氧气(21%),E组吸入高浓度氧气(50%~55%),每天1次,每次2 h,持续7 d。缺血前及缺血后第2、4、6、8天,取大鼠尾静脉血测量血尿素氮(BUN)及血肌酐(Cr)水平;术后第8天测量大鼠体重,并取右侧肾脏行病理检查。苏木精-伊红(HE)染色,以肾小管损伤评分评估肾损伤;免疫组织化学染色,以吸光度(A)值评估血红素氧化酶-1(HO-1)表达水平。BUN和Cr统计学分析采用重复测量方差分析,缺血前3组大鼠BUN及Cr比较采用单因素方差分析;体重、肾小管损伤评分、吸光度组间比较采用单因素方差分析。结果缺血前3组大鼠BUN及Cr水平未见明显差异,其中BUN分别为(8.99±0.35)、(8.87±0.28)、(9.07±0.78)mmol/L(F=0.259,P>0.05);Cr分别为(63.02±1.67)、(64.28±2.01)、(65.56±3.07)μmol/L(F=0.195,P>0.05)。术后第2、4、6、8天,S组大鼠BUN[(9.65±0.60)、(9.02±0.67)、(8.18±0.43)、(8.12±0.66)mmol/L]及Cr[(64.25±2.63)、(62.50±2.84)、(60.60±1.83)、(56.40±3.21)μmol/L]无明显变化;C组大鼠BUN[(24.47±2.06)、(52.91±1.38)、(32.69±1.76)、(13.99±1.38)mmol/L]及Cr[(215.16±3.63)、(265.16±5.25)、(208.69±6.18)、(157.02±6.83)μmol/L]与E组大鼠BUN[(15.65±1.19)、(35.64±2.13)、(16.36±0.55)、(10.72±1.37)mmol/L]及Cr[(164.98±2.88)、(214.40±3.55)、(123.45±3.85)、(100.80±3.92)μmol/L]逐渐升高,于第4天达高峰,后逐渐下降;其中,C组与E组大鼠BUN及Cr在上述时间点均显著高于S组,而E组大鼠BUN及Cr在上述Objective:To investigate the effect of normobaric hyperoxia on renal injury induced by ischemia-reperfusion in rats and its related mechanism.Methods:Eighteen adult male SD rats(Purchased from Liaoning Changsheng Biotechnology Co.,Ltd.)were enrolled and their left kidney was excised to establish a solitary kidney model.After one week,they were randomly assigned to 3 groups,6 rats in each group,namely sham-operated group(Group S),control group(Group C)and experimental group(Group E).In Group S,only the right renal pedicle was bluntly separated,but no ischemic treatment was given.Both Group C and Group E suffered from right renal ischemia for 40 minutes by clamping renal pedicle.After 24 hours,the three groups were all placed in a sealed oxygen chamber.Rats in Groups S and Group C inhaled normal concentration oxygen(21%),while rats in Group E inhaled high concentration oxygen(50%-55%),once a day for 2 hours each time for 7 days.Blood urea nitrogen(BUN)and creatinine(Cr)levels were measured by tail venous blood of rats before ischemia and on the 2nd,4th,6th and 8th days after ischemia.The body weight of rats was measured on the 8th day after operation,and hematoxylin-eosin(HE)staining and immunohistochemical staining were performed on the right kidney to observe the renal injury and heme oxygenase-1(HO-1)expression level.The experimental data were analyzed by SPSS 20.0 software.The measurement data were expressed as mean±standard deviation(Mean±SD).The statistical analysis of BUN and Cr used multiple measurement analysis of variance.The comparison of BUN and Cr in the three groups before ischemia was performed by one-way analysis of variance.The comparison of body weight,renal tubular injury score,and absorbance was performed by one-way analysis of variance.Results:There was no significant difference in BUN and Cr levels among the three groups before ischemia,BUN were(8.99±0.35),(8.87±0.28),(9.07±0.78)mmol/L;F=0.259,P>0.05);Cr were(63.02±1.67),(64.28±2.01),(65.56±3.07)μmol/L;F=0.195,P>0.05).On the 2nd,4th,6

关 键 词：缺血/再灌注损伤 高浓度吸氧 血红素氧化酶-1 

分 类 号：R692[医药卫生—泌尿科学]