外源褪黑素对硝酸盐胁迫下番茄幼苗生长及渗透调节物质的影响  

Effect of exogenous melatonin on tomato seedlings growth and osmotic adjustment substance under nitrate stress

作　　者：徐宁[1] 孙晓慧[1] 曹娜 王闯[1] 李光亚 薛淼云 XU Ning;SUN Xiaohui;CAO Na;WANG Chuang;LI Guangya;XUE Miaoyun(Liaocheng Vocational&Technical College,Liaocheng 252000,Shandong,China;Liaocheng University,Liaocheng 252059,Shandong,China;Vegetable Technology Promotion Center of Chiping County,Chiping 252100,Shandong,China)

机构地区：[1]聊城职业技术学院,山东聊城252000 [2]聊城大学,山东聊城252059 [3]山东省茌平县蔬菜技术推广中心,山东茌平252100

出　　处：《中国瓜菜》2020年第9期23-27,共5页China Cucurbits And Vegetables

基　　金：山东省重点科技攻关类研发计划(2019GNC21400,2019GNC21401);山东省科技厅重点研发计划(2017NC210011)。

摘　　要：为了探讨外源褪黑素调控番茄幼苗渗透调节物质抵御硝酸盐胁迫的生理机制,选用主栽番茄品种‘红粉冠军’为试验材料,采用灌根法研究不同浓度外源褪黑素对硝酸盐胁迫下番茄幼苗生长和叶片H-2O2含量、O2-产生速率、渗透调节物质含量的影响。结果表明,75 mmol·L^-1硝酸盐处理的番茄幼苗株高、茎粗、根长及幼苗地上部、地下部生物量均低于对照,胁迫处理下番茄幼苗叶片H-2O2含量和O2-产生速率一直比对照高,50、100、150μmol·L^-1外源褪黑素降低了H-2O2含量和O2-产生速率,显著缓解番茄幼苗膜脂过氧化,加速了番茄幼苗的生长。外源褪黑素处理的番茄幼苗叶片中脯氨酸、游离氨基酸、可溶性蛋白和可溶性糖含量显著提高,番茄幼苗渗透调节能力提升。综合分析表明,外源褪黑素提高了番茄幼苗叶片中渗透调节物质含量,有效缓解了硝酸盐对番茄幼苗细胞质膜的伤害,提升了番茄幼苗对硝酸盐胁迫的抗性,100μmol·L^-1外源褪黑素处理效果最显著。In order to explore the physiological mechanism of exogenous melatonin regulate osmotic adjustment substance on alleviating nitrate stress in tomato seedlings,the main tomato variety’Hongfen Champion’was used as the test material,the effects of different concentrations of exogenous melatonin on the tomato seedling growth and tomato seedling leaf H2O2 content,O2-production rate,osmotic adjustment substance content under nitrate stress through irrigation root method.The results showed that the plant height,stem diameter,root length,shoot biomass and root biomass of tomato seedlings were all lower than the control under the stress of 75 mmol·L^-1 nitrate.The H2O2 content and O2-production rate in tomato seedling leaves under stress were higher than that of control.The 50,100 and 150μmol·L^-1 exogenous melatonin reduced the H2O2 content and O2-production rate,significantly alleviated the membrane lipid peroxidation of tomato seedlings and accelerated the growth of tomato seedlings.The content of soluble sugar,soluble protein,proline and free amino acid in tomato leaves can be significantly increased by exogenous melatonin,and the osmotic regulation ability of tomato seedlings can be effectively enhanced.According to the comprehensive analysis,the addition of exogenous melatonin increased the content of osmotic regulatory substances in tomato seedling leaves,effectively alleviated the damage of nitrate to the cytoplasmic membrane of tomato seedlings,and improved the resistance of tomato seedlings to nitrate stress,the effect of exogenous melatonin at 100μmol·L^-1 was the most significant.

关 键 词：番茄 褪黑素 硝酸盐胁迫 渗透调节物质 

分 类 号：S641.2[农业科学—蔬菜学]