一个基于菲咯啉的Pb(Ⅱ)荧光探针  

A Novel Phenanthroline-Based Fluorescent Probe for Pb(Ⅱ)

作　　者：梅辉辉 杨铭阳 刘晓燕[1] 田玉平[1] 徐括喜[1] Mei Huihui;Yang Mingyang;Liu Xiaoyan;Tian Yuping;Xu Kuoxi(College of Chemistry and Chemical Engineering,Henan University,Kaifeng,Henan 475004;Mingsheng College,Henan University,Kaifeng,Henan 475004)

机构地区：[1]河南大学化学化工学院,河南开封475004 [2]河南大学民生学院,河南开封475004

出　　处：《有机化学》2020年第8期2508-2512,共5页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(No.31872311)资助项目。

摘　　要：设计并合成了一种基于菲咯啉的Pb^2+荧光探针L.在探针L的DMF-H2O(V:V=1:1,10 mmol/L Tris,pH=7.4)溶液中,加入Pb2+后导致其荧光猝灭,同时可以观察到溶液的颜色由黄色变为橙色,检测限低至2.68×10^-7 mol/L.通过紫外光谱、荧光光谱、高分辨质谱、核磁共振氢谱和计算拟合等手段研究了传感器L和Pb^2+离子的络合模式.进一步研究发现,L可以作为一种重复利用的检测Pb^2+荧光探针,并用于对活细胞中的Pb^2+的荧光成像.A novel fluorescent probe L for Pb^2+based phenanthroline derivative has been designed and synthesized.The probe L displayed fluorescence quenching emission excellent sensitivity specific toward Pb^2+ion.The introduction of Pb^2+ resulted the quenching fluorescence of probe L and the color of the solution can be observed changes from yellow to orange in DMF-H2O(V:V=1:1,10 mmol/L Tris,pH=7.4).The detection limit was as low as 2.68×10^-7 mol/L.The bonding mechanism was confirmed by UV-vis,fluorescence,HRMS,1H NMR experiments and theoretical calculation.It was found that the probe had excellent reversibility for detection of Pb^2+ and could be used to effectively identify Pb^2+ in living cells.

关 键 词：菲咯啉衍生物 Pb2+荧光探针 活细胞成像 

分 类 号：O657.3[理学—分析化学]