Global identification of genes associated with xylan biosynthesis in cotton fiber  

作　　者：CHEN Feng GUO Yanjun CHEN Li GAN Xinli LIU Min LI Juan XU Wenliang 

机构地区：[1]Hubei Key Laboratory of Genetic Regulation and Integrative Biology,School of Life Sciences,Central China Normal University,Wuhan 430079,China [2]Zhengzhou Research Base,State Key Laboratory of Cotton Biology,Zhengzhou University,Zhengzhou 450001,China

出　　处：《Journal of Cotton Research》2020年第3期184-198,共15页棉花研究（英文）

基　　金：National Natural Science Foundation of China,China[grant numbers:31671735;31970516];Hubei provincial Natural Science Foundation,China[grant number:2016CFA071];self-determined research funds of Central China Normal University from the colleges’basic research and operation of Ministry of Education,China(CCNU18TS021).

摘　　要：Background:Mature cotton fiber secondary cell wall comprises largely of cellulose(>90%)and small amounts of xylan and lignin.Little is known about the cotton fiber xylan biosynthesis by far.Results:To comprehensively survey xylan biosynthetic genes in cotton fiber,we identified five IRX9,five IRX10,one IRX14,six IRX15,two FRA8,one PARVUS,eight GUX,four GXM,two RWA,two AXY9,13 TBL genes by using phylogenetic analysis coupled with expression profile analysis and co-expression analyses.In addition,we also identified two GT61 members,two GT47 members,and two DUF579 family members whose homologs in Arabidopsis were not functionally characterized.These 55 genes were regarded as the most probable genes to be involved in fiber xylan biosynthesis.Further complementation analysis indicated that one IRX10 like and two FRA8 related genes were able to partially recover the irregular xylem phenotype conferred by the xylan deficiency in their respective Arabidopsis mutant.We conclude that these genes are functional orthologs of respective genes that are implicated in GX biosynthesis.Conclusion:The list of 55 cotton genes presented here provides not only a solid basis to uncover the biosynthesis of xylan in cotton fiber,but also a genetic resource potentially useful for future studies aiming at fiber improvement via biotechnological approaches.

关 键 词：Cotton fiber Secondary cell wall Xylan biosynthesis Expression profile CO-EXPRESSION 

分 类 号：S562[农业科学—作物学]