A genome-wide identification of the BLH gene family reveals BLH1 involved in cotton fiber development  

作　　者：LIU Cuixia LI Zhifang DOU Lingling YUAN Yi ZOU Changsong SHANG Haihong CUI Langjun XIAO Guanghui 

机构地区：[1]Key Laboratory of the Ministry of Education for Medicinal Plant Resources and Natural Pharmaceutical Chemistry,National Engineering Laboratory for Resouce Development of Endangered Crude Drugs in the Northwest of China,College of Life Sciences,Shaanxi Normal University,Xi’an 710119,Shaanxi,China [2]College of Biology Pharmacy and Food Engineering,Shangluo University,Shangluo 726000,Shaanxi,China [3]State Key Laboratory of Cotton Biology,State Key Laboratory of Crop Stress Adaptation and Improvement,School of Life Sciences,Henan University,Kaifeng 475001,Henan,China [4]School of Chemistry and Chemical Engineering,Xianyang Normal University,Xianyang 712000,Shaanxi,China [5]Zhengzhou Research Base,State Key Laboratory of Cotton Biology,Zhengzhou University,Zhengzhou 450001,China

出　　处：《Journal of Cotton Research》2020年第3期199-209,共11页棉花研究（英文）

基　　金：the State Key Laboratory of Cotton Biology Open Fund(CB2019A03,2019A09 and CB2020A12);the National Natural Science Foundation of China(31872175);the Fundamental Research Funds for the Central Universities(GK202002005 and GK202001004);Natural Science Basic Research Plan in Shaanxi Province of China(2018JZ3006 and 2019JQ-062),Shaanxi Youth Entrusted Talents Program(20190205),Shaanxi Postdoctoral Project(2018BSHYDZZ76);Young Elite Scientists Sponsorship Program by CAST(2019-2021QNRC001).

摘　　要：Background:Cotton is the world’s largest and most important source of renewable natural fiber.BEL1-like homeodomain(BLH)genes are ubiquitous in plants and have been reported to contribute to plant development.However,there is no comprehensive characterization of this gene family in cotton.In this study,32,16,and 18 BLH genes were identified from the G.hirsutum,G.arboreum,and G.raimondii genome,respectively.In addition,we also studied the phylogenetic relationships,chromosomal location,gene structure,and gene expression patterns of the BLH genes.Results:The results indicated that these BLH proteins were divided into seven distinct groups by phylogenetic analysis.Among them,25 members were assigned to 15 chromosomes.Furthermore,gene structure,chromosomal location,conserved motifs,and expression level of BLH genes were investigated in G.hirsutum.Expression profiles analysis showed that four genes(GhBLH1_3,GhBLH1_4,GhBLH1_5,and GhBLH1_6)from BLH1 subfamily were highly expressed during the fiber cell elongation period.The expression levels of these genes were significantly induced by gibberellic acid and brassinosteroid,but not auxin.Exogenous application of gibberellic acid significantly enhanced GhBLH1_3,GhBLH1_4,and GhBLH1_5 transcripts.Expression levels of GhBLH1_3 and GhBLH1_4 genes were significantly increased under brassinosteroid treatment.Conclusions:The BLH gene family plays a very important role in many biological processes during plant growth and development.This study deepens our understanding of the role of the GhBLH1 gene involved in fiber development and will help us in breeding better cotton varieties in the future.

关 键 词：COTTON G.hirsutum BLH gene family Fiber development 

分 类 号：S562[农业科学—作物学]