混合丛枝菌根真菌接种时期对滇重楼幼苗内源激素含量的影响  

Effect of inoculation time of arbuscular mycorrhizal fungi on endogenous hormone content in Paris polyphylla var. yunnanensis seedlings

作　　者：杨玲 杨敏 郭冬琴 潘兴娇 张杰 张蓝月 周浓 YANG Ling;YANG Min;GUO Dong-qin;PAN Xing-jiao;ZHANG Jie;ZHANG Lan-yue;ZHOU Nong(Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of Three Gorges Reservoir Area’s Medicinal Herbs,College of Food and Biology Engineering,Chongqing Three Gorges University,Chongqing 404120,China;College of Pharmacy and Chemistry,Dali University,Dali 671000,China)

机构地区：[1]重庆三峡学院生物与食品工程学院,三峡库区道地药材绿色种植与深加工重庆市工程实验室,重庆404120 [2]大理大学药学与化学学院,云南大理671000

出　　处：《中草药》2020年第13期3535-3544,共10页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(81260622);重庆市自然科学科学基金项目资助(CSTC2018jcyjAX0267,CSTC2015jcyjA10110)。

摘　　要：目的研究混合丛枝菌根(AM)真菌不同接种时期对滇重楼Paris polyphylla Smith var. yunnanensis幼苗内源激素含量的影响。方法室内盆栽法,分别将滇重楼种子、1年生苗、2年生苗接种混合AM真菌,于灭菌土壤基质中进行共生培养,采用高效液相色谱法测定内源激素玉米素核苷(ZR)、赤霉素(GA)、吲哚乙酸(IAA)和脱落酸(ABA)含量。结果混合AM真菌对不同时期,不同种源的滇重楼根状茎和根中内源激素ZR、GA、IAA、ABA含量变化的影响不尽相同。整体上,与对照组比较,野生种源和栽培种源滇重楼根状茎和根的ZR和GA内源激素含量均呈增加趋势,ABA含量均呈减少趋势。滇重楼根状茎和根中IAA/ABA、GA/ABA、ZR/ABA增大。结论 1年生苗期接种S2、S6型混合AM真菌,对促进滇重楼幼苗根状茎和根生长的综合效果最佳。Objective To study the effect of the inoculation time of arbuscular mycorrhizal(AM) fungi on endogenous hormone content in Paris polyphylla var. yunnanensis seedlings. Methods The indoor pot test was applied. The seeds, one-year-old seedlings, and two-year-old seedlings of P. polyphylla var. yunnanensis were inoculated by different mixed AM fungi and cultivated in the sterilized soil matrix respectively. The contents of endogenous hormone zeatin riboside(ZR), gibberellin(GA), indole acetic acid(IAA) and abscisic acid(ABA) were determined by HPLC. Results The results showed that the endogenous hormone(ZR, GA, IAA and ABA) contents in rhizome and fibrous roots of P. polyphylla var. yunnanensis from different regions or different time inoculated by mixed AM fungi were various. As a whole, the contents of endogenous hormone ZR and GA increased obviously in the rhizome and fibrous roots of the wild and cultivated P. polyphylla var. yunnanensis compared with the control group(CK), while the content of ABA reduced. The IAA/ABA value, GA/ABA value and ZR/ABA value increased obviously in the rhizome and fibrous roots of P. polyphylla var. yunnanensis. Conclusion The one-year-old of P. polyphylla var. yunnanensis inoculated with S2 and S6 mixed AM fungi showed the best comprehensive effect for promoting growth of the rhizome and fibrous roots of P. polyphylla var. yunnanensis seedlings.

关 键 词：滇重楼 丛枝菌根真菌 玉米素核苷 赤霉素 吲哚乙酸 脱落酸 

分 类 号：R282.2[医药卫生—中药学]