铁皮石斛叶的化学成分研究  

Phytochemical investigation of leaves of Dendrobium officinale

作　　者：任刚 陈优婷 叶金宝 钟国跃 肖川云 邓文赞 陈云龙[2] REN Gang;CHEN You-ting;YE Jin-bao;ZHONG Guo-yue;XIAO Chuan-yun;DENG Wen-zan;CHEN Yun-long(Research Center of Natural Resources of Chinese Medicinal Materials and Ethnic Medicine,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;College of Life Sciences,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]江西中医药大学中药资源与民族药研究中心,江西南昌330004 [2]浙江大学生命科学学院,浙江杭州310058

出　　处：《中草药》2020年第14期3637-3644,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金(81873086);江西中医药大学一流学科(中药学)科研启动基金项目(JXSYLXK-ZHYAO027);江西省百千万人才工程资助[赣人社字(2016023号)];浙江省教育厅基金(Y201122277)。

摘　　要：目的研究兰科植物铁皮石斛Dendrobiumofficinale叶的化学成分。方法运用Sephadex LH-20、MCI CHP-20P、ODS等柱色谱和高效液相色谱技术进行分离纯化,并根据理化性质和NMR、MS波谱数据鉴定化合物结构。结果从铁皮石斛叶中分离得到24个化合物,分别鉴定为3,4-二羟基-5,4′-二甲氧基联苄(1)、杓唇石斛素(2)、4,4′-二羟基-3,5-二甲氧基联苄(3)、densiflorol A(4)、(S)-3,4,α-三羟基-5,4′-二甲氧基联苄(5)、石斛酚(6)、dendrocandin U(7)、dendrocandin B(8)、黑麦草内酯(9)、(6R,9S)-6,9-二羟基-4,7-巨豆二烯-3-酮-9-氧-β-D-吡喃葡萄糖苷(10)、(6R,9S)-9-羟基-4,7-巨豆二烯-3-酮-9-氧-β-D-吡喃葡萄糖苷(11)、(+)-丁香脂素(12)、芦丁(13)、2-羟基苯并噻唑(14)、对羟基苯乙酮(15)、对羟基苯甲酸(16)、原儿茶酸(17)、儿茶酚(18)、对羟基苯丙酸乙酯(19)、甘油亚麻酸酯(20)、亚麻酸2-丁氧基乙酯(21)、棕榈酸(22)、十八碳癸二烯酸-2,3-二羟基丙酯(23)和urticifolene(24)。结论化合物10、11、14~23为首次从兰科植物中分离得到,化合物1、2、4和6为首次从该植物中分离得到。Objective To investigate the chemical constituents of leaves of Dendrobiumofficinale. Methods Compounds were isolated from the leaves of D.officinale by column chromatography over Sephadex LH-20, MCI GEL CHP-20 P, and ODS as well as by preparative HPLC. Their structures were identified by the analysis of their physical and chemical properties and the spectra data of NMR and MS. Results Twenty-four compounds were isolated from the leaves of the plant, namely 3,4-dihydroxy-5,4′-dimethoxy bibenzyl(1), moscatilin(2), 4,4′-dihydroxy-3,5-dimethoxybibenzyl(3), densiflorol A(4),(S)-3,4,α-trihydroxy-5,4′-dimethoxybibenzyl(5), gigantol(6), dendrocandin U(7), dendrocandin B(8), loliolide(9),(6 R,9 S)-dihydroxy-megastigma-4,7-dien-3-one-9-O-β-D-glucopyranoside(10),(6 R,9 S)-9-hydroxy-megastigma-4,7-dien-3-one-9-O-β-D-glucopyranoside(11),(+)-syringaresinol(12), rutin(13), 2-benzothiazolol(14), p-hydroxyacetophenone(15), p-hydroxyl-benzoic acid(16), protocatechuic acid(17), catechol(18), ethyl p-hydroxyhydrocinnamate(19), 1-glycerol linolenate(20), 2-butoxyethyl linolenate(21), palmitic acid(22), octadecadienoic acid-2,3-dihydroxypropyl ester(23), and urticifolene(24). Conclusion Itis the first report of the occurrence of compounds 10, 11, 14—23 in Orchidaceae family. Compounds 1, 2, 4, and 6 are found in D. officinale for the first time.

关 键 词：兰科 石斛属 铁皮石斛 联苄 杓唇石斛素 石斛酚 (6R 9S)-9-羟基-4 7-巨豆二烯-3-酮-9-氧-β-D-吡喃葡萄糖苷 

分 类 号：R284.1[医药卫生—中药学]