基于网络药理学的三七传统功效作用机制研究  

Study on traditional effect of Panax notoginseng based on network pharmacology

作　　者：武琦 林娟[4] 刘耀晨 韩彦琪[2,3] 许浚[2,3] 龚苏晓[2,3] 张洪兵[2,3] 张铁军[2,3] 郭海彪[4] WU Qi;LIN Juan;LIU Yao-chen;HAN Yan-qi;XU Jun;GONG Su-xiao;ZHANG Hong-bing;ZHANG Tie-jun;GUO Hai-biao(Tianjin Medical University,Tianjin 300070,China;National&Local United Engineering Laboratory of Modern Preparation and Quality Control Technology of Traditional Chinese Medicine,Tianjin Institute of Pharmaceutical Research,Tianjin 300462,China;Tianjin Key Laboratory of Quality Marker of Traditional Chinese Medicine,Tianjin Institute of Pharmaceutical Research,Tianjin 300462,China;Guangzhou Baiyunshan Hutchison Whampoa Chinese Medicine Co.,Ltd,Guangzhou 510515,China)

机构地区：[1]天津医科大学,天津300070 [2]天津药物研究院,中药现代制剂与质量控制技术国家地方联合工程实验室,天津300462 [3]天津药物研究院,天津市中药质量标志物重点实验室,天津300462 [4]广州白云山和记黄埔中药有限公司,广东广州510515

出　　处：《中草药》2020年第14期3717-3727,共11页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金重点项目(81830111);天津市自然科学基金项目(19JCQNJC12700)。

摘　　要：目的探索三七活血止血、消肿止痛传统功效的网络调控机制。方法选取三七药材中12个入血成分为研究对象,依据反向分子对接的方法预测化合物靶点,借助String 10数据库与Omicsbean在线分析软件对靶点进行信号通路分析、基因本体(gene ontology,GO)功能富集分析,利用Cytoscape软件构建网络。结果 12个化合物(三七皂苷R1、人参皂苷Rg1、人参皂苷Re、人参皂苷Rh1、人参皂苷Rg2、人参皂苷Rb1、人参皂苷Rd、人参皂苷F2、人参皂苷Rg3、人参皂苷Rk1、三七素、槲皮素)可通过65个相关靶点作用于65条信号通路,主要涉及抑制血栓生成、纤溶、血管新生、舒张血管、凝血、抗炎以及镇痛等方面,进一步得到三七"化合物-靶点-通路-药理作用-功效"的网络药理图。结论三七通过作用于F2、F10、PLAT、VEGFA、NOS2、IL6、PTGES、OPRD1等关键蛋白干预了多个与活血止血、消肿止痛相关的生物过程,初步揭示了其传统功效的作用机制。Objective To explore the network regulation mechanism of blood-activating and hemostatic and detumescent and analgesic traditional effects of Panax notoginseng. Methods Targets of the 12 components of P. notoginseng absorbed in plasma were predicted according to the reverse pharmacophore method. Gene ontology(GO) function enrichment and pathway analysis of the targets were analyzed by Omicsbean online analysis software and String 10 database. Finally, Cytoscape software was used to construct the network pharmacology map. Results A total of 12 compounds(notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rh1, ginsenoside Rg2, ginsenoside Rb1, ginsenoside Rd, ginsenoside F2, ginsenoside Rg3, ginsenoside Rk1, dencichine and quercetin) affected 65 pathways through 65 related targets, which were associated with anti-thrombosis, fibrinolysis, angiogenesis, vasodilation, blood coagulation, anti-inflammation and analgesia. The network of "compound-target-pathway-pharmacological action-efficacy" was also constructed. Conclusion P. notoginseng interferes with multiple biological processes related to activating blood circulation, hemostasis, detumescence and analgesia by acting on several key proteins such as F2, F10, PLAT, VEGFA, NOS2, IL6, PTGES, OPRD1, etc.

关 键 词：三七 网络药理学 活血 止血 消肿 止痛 三七皂苷R1 人参皂苷Rg1 人参皂苷RE 人参皂苷RH1 人参皂苷RG2 人参皂苷Rb1 人参皂苷RD 人参皂苷F2 人参皂苷Rg3 人参皂苷Rk1 三七素 槲皮素 

分 类 号：R285.5[医药卫生—中药学]