不同产地黄秋葵中5种黄酮成分HPLC分析  

Content determination of five flavonoids in Abelmoschus esculentus from different habitats by HPLC

作　　者：唐云[1] 李国鹏 殷启霖 易伟 曾皓 李伟[1] TANG Yun;LI Guo-peng;YIN Qi-lin;YI Wei;ZENG Hao;LI Wei(Jiangxi Key Laboratory of Natural Active Pharmaceutical Ingredients,Yichun University,Yichun 336000,China;Institute of Forensic Science,Public Security Bureau of Yichun,Yichun 336000,China;Shandong Buchang Pharmaceuticals Co.,Ltd.,Heze 274000,China)

机构地区：[1]宜春学院江西省天然活性成分重点实验室,江西宜春336000 [2]宜春市公安局刑事科学技术研究所,江西宜春336000 [3]山东步长制药股份有限公司,山东菏泽274000

出　　处：《中草药》2020年第14期3797-3801,共5页Chinese Traditional and Herbal Drugs

基　　金：江西省教育厅科学技术研究项目(GJJ151024);江西省教育厅科学技术研究项目(GJJ161040)。

摘　　要：目的建立HPLC法测定12个不同产地黄秋葵中槲皮素-3-O-龙胆二糖苷、槲皮素-3-O-芸香糖苷、异槲皮苷、槲皮素、5,7,4′-三羟基黄酮的含量,为其合理开发利用提供参考。方法采用COSMOSIL色谱柱(250 mm×4.6 mm,5.4μm);以乙腈和0.2%的磷酸水溶液为流动性进行梯度洗脱,检测波长为353nm,体积流量1mL/min。结果槲皮素-3-O-龙胆二糖苷、槲皮素-3-O-芸香糖苷、异槲皮苷、槲皮素、5,7,4′-三羟基黄酮分别在15.72~251.50μg/mL(r=0.999 5)、15.47~247.50μg/mL(r=0.999 6)、14.41~230.50μg/mL(r=0.999 5)、21.88~350.00μg/mL(r=0.999 7)、17.25~276.00μg/mL(r=0.999 1)浓度范围内呈良好的线性关系;平均加样回收率分别为97.46%、96.74%、100.21%、95.66%、98.35%,RSD为1.97%、1.37%、1.86%、2.72%、2.23%。结论方法简单易行、准确度高,适合于同时测定槲皮素-3-O-龙胆二糖苷、槲皮素-3-O-芸香糖苷、异槲皮苷、槲皮素、5,7,4′-三羟基黄酮的含量,可作为黄秋葵质量评价的方法之一。Objective To establish a method for determination of quercetin-3-O-gentiobioside, quercetin-3-O-rutinoside, isoquercitrin, quercetin, and 5,7,4′-trihydroxyflavone in Abelmoschus esculentus from 12 different regions of China. Methods COSMOSIL column(250 mm × 4.6 mm, 5.4 μm) was used. The gradient elution was carried out with acetonitrile and 0.2% phosphoric acid aqueous solution. The detection wavelength was 353 nm and the flow rate was 1 mL/min. Results The contents of quercetin-3-O-gentiobioside, quercetin-3-O-rutinoside, isoquercitrin, quercetin and 5,7,4′-trihydroxyflavone had good linear relationship in the ranges of 15.72— 251.50 μg/mL(r = 0.999 5), 15.47—247.50 μg/mL(r = 0.999 6), 14.41—230.50 μg/mL(r = 0.999 5), 21.88—350.00 μg/mL(r = 0.999 7), and 17.25—276.00 μg/mL(r = 0.999 1). The average sample recovery rate was 97.46%, 96.74%, 100.21%, 95.66%, 98.35%, and RSD was 1.97%, 1.37%, 1.86%, 2.72%, 2.23%, respectively. Conclusion This method is simple and accurate. It is suitable for simultaneous determination of quercetin-3-O-gentiobioside, quercetin-3-O-rutinoside, isoquercitrin, quercetin and 5,7,4′-trihydroxyflavone. The content of the five flavones can be used as one of the methods for evaluating the quality of A. esculentus.

关 键 词：黄秋葵 黄酮 HPLC 槲皮素-3-O-龙胆二糖苷 槲皮素-3-O-芸香糖苷 异槲皮苷 槲皮素 5 7 4′-三羟基黄酮 

分 类 号：R286.2[医药卫生—中药学]