江苏部分地区鸡源与猪源弯曲菌耐药性分析与毒力基因检测  

Antimicrobial Resistance and Virulence Genes of Campylobacter spp.Isolated from Chicken and Swine Farms in Jiangsu Province

作　　者：唐梦君[1,2] 周倩 张小燕[1,2] 张静 唐修君[1,2] 陆俊贤 周生[1] 蒲俊华 高玉时[1,2] TANG Mengjun;ZHOU Qian;ZHANG Xiaoyan;ZHANG Jing;TANG Xiujun;LU Junxian;ZHOU Sheng;PU Junhua;GAO Yushi(Jiangsu Institute of Poultry Science,Yangzhou 225125,China;Key Laboratory for Poultry Genetics and Breeding of Jiangsu Province,Yangzhou 225125,China)

机构地区：[1]江苏省家禽科学研究所,扬州225125 [2]江苏省家禽遗传育种重点实验室,扬州225125

出　　处：《畜牧兽医学报》2020年第9期2284-2292,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学青年基金(31700005);江苏现代农业(肉鸡)产业技术体系(JATS[2019]382);江苏省现代农业—重点及面上项目(BE2018363);江苏省属公益类科研院所自主科研经费(BM2018026);江苏省家禽遗传育种重点实验室资助项目(JQLAB-202002)。

摘　　要：旨在了解江苏省部分地区鸡源与猪源弯曲菌的耐药及毒力基因携带情况。从江苏省25个规模养殖场采集250份粪便样品进行弯曲菌的分离鉴定,采用琼脂平板稀释法测定9种抗菌药物的最小抑菌浓度(minimal inhibitory concentrations,MICs),PCR方法扩增弯曲菌8种与致病力相关的毒力基因。结果显示:共分离得到93株弯曲菌,包括空肠弯曲菌45株,结肠弯曲菌48株;空肠弯曲菌对萘啶酸(80.0%)、四环素(71.1%)和环丙沙星(66.7%)的耐药率较高,而结肠弯曲菌对红霉素(87.5%)、萘啶酸(79.2%)和阿奇霉素(72.9%)产生较强的耐药性,分离株多重耐药现象严重,多重耐药率达67.7%;8个毒力基因在弯曲菌分离株中的携带率不同,cdtB和cadF携带率为100%,htrB为97.8%,clpP为76.3%,csrA为18.3%,wlaN为5.4%,cstⅡ为2.2%,cgtB为0%。结果提示,江苏省畜禽养殖场弯曲菌分离株多重耐药情况严重,毒力相关基因在弯曲菌中分布广泛。The aim of this study was to investigate the antimicrobial resistance,virulence genes of Campylobacter spp.isolated from chicken and swine farms in Jiangsu Province.Campylobacter spp.were isolated and identified from two hundred and fifty fecal samples collected from twenty-five animal farms in Jiangsu Province.Campylobacter strains were tested for the antimicrobial susceptibility against to 9 kinds of antimicrobial agents by using an agar dilution method.Eight virulence genes(cdtB,cadF,htrB,clpP,csrA,wlaN,cstⅡ,and cgtB)were detected by polymerase chain reaction.Ninety-three Campylobacter strains were isolated and identified from 250 samples,including 45 C.jejuni strains and 48 C.coli strains.The highest percentage of antimicrobial resistance was found for nalidixic acid(80.0%),tetracycline(71.1%)and ciprofloxacin(66.7%)in C.jejuni isolates.The C.coli isolates were most frequently resistant to erythromycin(87.5%),nalidixic acid(79.2%)and azithromycin(72.9%).Moreover,the multi-drug resistance(resistance to three or more antimicrobial classes)was common among Campylobacter isolates with a rate of 67.7%.On the other hand,the 93 Campylobacter strains showed a wide variation for the presence of the 8 virulence genes,cdtB and cadF were positive for all isolates,while htrB,clpP,csrA,wlaN,cstⅡand cgtB was 97.8%,76.3%,18.3%,5.4%,2.2%,and 0%,respectively.The results indicated that the multiple drug resistance of Campylobacter strains from animal origin was relatively serious.In addition,the virulence-associated genes were detected widely among Campylobacter strains.

关 键 词：弯曲菌 耐药性 毒力基因 分离鉴定 

分 类 号：S852.613[农业科学—基础兽医学]