肝脏组织的相位衬度成像实验研究  

Experiment Investigation of Liver Phase Contrast Imaging

作　　者：刘安娜 苏雅 马紫瑶 房蕾 彭屹峰[1] 肖体乔[2] 杜国浩[2] 何伟[3] LIU An-na;SU Ya;MA Zi-yao;FANG Lei;PENG Yi-feng;XIAO Ti-qiao;DU Guo-hao;HE Wei(Department of Radiology,Putuo Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200062,P.R.C.;Shanghai institute of Applied Physics,Chinese Academy of Science;Institute of High Energy Physics,Chinese Academy of Science)

机构地区：[1]上海中医药大学附属普陀医院放射科,上海200062 [2]中国科学院上海应用物理研究所 [3]中国科学院北京高能物理研究所同步辐射实验室

出　　处：《中国医学计算机成像杂志》2020年第4期380-383,共4页Chinese Computed Medical Imaging

基　　金：上海市自然科学基金,NO:11ZR1427800。

摘　　要：目的:利用同步辐射光源的特性,对大鼠的肝脏组织分別进行类同轴成像、衍射增强成像、干涉法成像及光栅成像等4种相位衬度方法的成像实验,通过4种技术不同成像效果的比较、分析,评价同步辐射相位衬度成像方法的意义以及临床应用的前景。方法:在同步辐射光源装置,分别根据成像机制设计4种不同的光路进行成像,均采用经过福尔马林固定的大鼠离体肝脏样品,根据光路及成像方法不同分別调节曝光参数以达到最佳成像效果。结果:4种成像技术均获得良好的成像效果,分辨率均能达到微米(μm)量级,均具有独特的成像优点,类同轴成像、干涉法成像、光栅成像、衍射增强成像在对肝脏样品的血管显示上具有较大的优势,可以显示到到远端(8级)血管,除此之外,类同轴成像技术显示肝脏实质结构上优势较为明显,肝小叶结构清晰显示,包括小叶间结构及窦状间隙血管.肝细胞在围绕窦状间隙中呈板状排列。结论:相位衬度成像方法具有较高的组织衬度及较好的图像分辨率,能够显示包括肝小叶在内的微小结构或多级血管分支结构,对于肝脏肿瘤生长过程中肿瘤新生血管的形态、分布以及肝脏组织细胞微观层面的研究,有望在肝脏的生理、病理及病变机制的可视化研究上获得较大的突破。Purpose:With the outstanding characters of SR light,the rat livers imaging with four different phase contrast imaging(PCI)including in line imaging,diffraction enhanced imaging(DEI),interfere imaging and grating were performed and compared.The significance of SR imaging was evaluated.Methods:There were four setups,in which the liver samples were parted and imaged.Results:All of the four imaging techniques achieved good imaging results with resolutions up to the jim level.They were all with great advantages in displaying the blood vessels in liver samples,the distal blood vessels(8 level branches)could be shown.In-line imaging was with great advantages in displaying the structure of liver parenchyma.Conclusion:The phase contrast imaging(PCI)is with good contrast and resolution,has great advantages in display of minute structure including hepatic lobule and vessels.It is hopeful with breakthrough in hepatic physiology,pathology and disease mechanism in the future.

关 键 词：肝脏 同步辐射 相位衬度成像 大鼠 

分 类 号：O433[机械工程—光学工程]