FADS2对鸡前脂肪细胞脂肪酸代谢相关基因表达的影响  

Effect of FADS2 on the expression of genes related to fatty acid metabolism in chicken preadipocytes

作　　者：欧小倩 王莹 何程实 李春晖 刘国庆 杨少华 OU Xiaoqian;WANG Ying;HE Chengshi;LI Chunhui;LIU Guoqing;YANG Shaohua(School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)

机构地区：[1]合肥工业大学食品与生物工程学院,安徽合肥230601

出　　处：《合肥工业大学学报（自然科学版）》2020年第9期1269-1275,共7页Journal of Hefei University of Technology：Natural Science

基　　金：安徽省皖江禽产业研究院公共服务平台资助项目(1401032006)。

摘　　要：脂肪酸的含量及比例对改善鸡肉品质和风味起着重要作用,因此探究调控脂肪酸代谢的关键基因和作用机制能够为改善鸡肉品质提供新的科学依据。基于前期RNA-seq结果,确定脂肪酸脱氢酶2(FADS2)为影响鸡不饱和脂肪酸比率的关键基因。文章构建FADS2基因RNA干扰慢病毒表达载体,转染鸡前脂肪细胞并鉴定干扰效率,筛选出有效干扰片段并进行正式试验,通过设置阴性对照组,转录组测序检测相关基因表达。结果表明,190个基因显著差异性表达,其中65个基因下调,125个基因上调。通过比较基因组学和基因功能分析,鉴定出STAT1、LY86、FABP5、OGFR、ACTA1、SLC5A6、NCOA7、KLF2、KLF4、ACSL5和FABP3基因可能是FADS2基因影响脂肪酸代谢的相关下游基因,为深入研究脂肪酸代谢过程提供一定的理论基础。The content and proportion of fatty acids play an important role in improving the quality and flavor of chickens.Therefore,exploring the key genes and mechanism regulating fatty acid metabolism can provide a new scientific basis for improving the quality of chicken.Based on the previous RNA-seq results,fatty acid desaturase 2(FADS2)was identified as a key gene affecting the ratio of unsaturated fatty acids in chickens.On this basis,the FADS2 gene RNA interference lentiviral expression vector was constructed,and transfected into chicken preadipocytes to identify the interference efficiency.The effective interference fragment and the negative control group were screened and transfected into chicken preadipocytes,respectively.Qualified RNA extracted from cells was sequenced by transcriptome sequencing and a total of 190 genes were detected,of which 65 genes were down-regulated and 125 genes were up-regulated.By comparative genomics and gene function analysis,STAT1,LY86,FABP5,OGFR,ACTA1,SLC5A6,NCOA7,KLF2,KLF4,ACSL5 and FABP3 were thought to be related downstream genes of FADS2 gene affecting fatty acid metabolism,which provides a theoretical basis for further study of fatty acid metabolism.

关 键 词：FADS2基因 鸡前脂肪细胞 转录组测序 差异表达基因 下游基因 

分 类 号：Q756[生物学—分子生物学]