白藜芦醇调控miR-409-3p/FKBP14抑制胃癌细胞BGC823恶性进程  

Resveratrol regulates miR-409-3p/FKBP14 to inhibit the malignant biological processes of gastric cancer BGC823 cells

作　　者：吴懿[1] 朱阿考[1] 单毓强[1] 应荣超[1] 金慧成[1] WU Yi;ZHU Akao;SHAN Yuqiang;YING Rongchao;JIN Huicheng(Hangzhou First People's Hospital,Gastrointestinal and Anal Surgery,Hangzhou 310006,Zhejiang,China)

机构地区：[1]杭州市第一人民医院胃肠肛外科,浙江杭州310006

出　　处：《中国临床药理学与治疗学》2020年第9期968-975,共8页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省自然科学基金/探索项目(LQ20H160017)。

摘　　要：目的:探究白藜芦醇(resveratrol,RES)调控miR-409-3p/FKBP14对胃癌(gastric cancer,GC)细胞恶性生物学进程的影响。方法:CCK-8实验研究RES对BGC823细胞活性的影响;qRT-PCR检测RES对miR-409-3p表达的调控;EdU实验研究RES对BGC823细胞增殖的影响;Transwell实验研究RES对BGC823细胞迁移和侵袭的影响;双荧光素酶报告基因和Western blot实验检测miR-409-3p对FKBP14表达的调控。结果:RES(>200μmol/L)能够显著抑制BGC823细胞活性。RES通过上调miR-409-3p抑制胃癌细胞增殖、迁移和侵袭。FKBP14是miR-409-3p的靶基因,RES能够通过上调miR-409-3p抑制胃癌细胞中FKBP14的表达。过表达FKBP14后减弱RES的抑癌作用。结论:RES可通过调控miR-409-3p/FKBP14信号通路在胃癌中发挥肿瘤抑制作用,RES有应用于临床胃癌治疗的潜力。AIM:To investigate the effect of resveratrol(RES)on the malignant biological processes of gastric cancer(GC)cells.METHODS:CCK-8 assay was used to study the effect of RES on the viability of BGC823 cells;qRT-PCR was used to detect the regulation of RES on the expression of miR-409-3p;EdU assay was used to study the effect of RES on the proliferation of BGC823 cells;transwell assay was used to study the effect of RES on the migration and invasion of BGC823 cells;luciferase reporter assay and western blot assay were used to study the regulation of miR-409-3p on the expression of FKBP14.RESULTS:RES(>200μmol/L)significantly inhibited the viability of BGC823 cells.RES inhibited the proliferation,migration and invasion of gastric cancer cells by up-regulating miR-409-3p.FKBP14 was the target gene of miR-409-3p.Res could inhibit the expression of FKBP14 in gastric cancer cells by up-regulating miR-409-3p.Overexpression of FKBP14 attenuated the antitumor effect of RES.CONCLUSION:RES can suppress the tumor progression in gastric cancer by regulating miR-409-3p/FKBP14 signaling pathway,indicating the potential value of RES in clinical treatment of gastric cancer.

关 键 词：白藜芦醇 胃癌 miR-409-3p 

分 类 号：R965.2[医药卫生—药理学]