‘芽黄’红瑞木炭疽病病原菌的分子鉴定  

Molecular identification of Colletotrichum species causing anthracnose disease of Cornus alba‘Bud’s Yellow’

作　　者：罗卿权 LUO Qingquan(Shanghai Academy of Landscape Architecture Science and Planning, Institute of Plant Protection, Shanghai 200232, China;Shanghai Engineering Research Center of Landscaping on Challenging Urban Sites, Shanghai 200232, China)

机构地区：[1]上海市园林科学规划研究院植物保护研究所,上海200232 [2]上海市困难立地绿化工程技术研究中心,上海200232

出　　处：《植物保护》2020年第5期102-109,共8页Plant Protection

基　　金：国家林业和草原局科技发展中心生物安全与遗传资源管理项目(KJZXSA2019027)。

摘　　要：‘芽黄’红瑞木炭疽病在上海普遍发生。本研究从上海迪士尼园区采集疑似炭疽病的红瑞木病叶,进行了分离培养,获得2种培养形态的炭疽菌,致病性测定证明其为致病菌。联合核糖体转录间隔区、3-磷酸甘油醛脱氢酶、钙调蛋白和β-微管蛋白2多基因序列,使用最大似然法和贝叶斯分析法分别构建了多基因系统进化树。结果显示,分离获得的2株炭疽菌菌株分别与暹罗炭疽菌Colletotrichum siamense和胶孢炭疽菌C.gloeosporioides聚集在一个分支,辅以形态学特征,鉴定引起‘芽黄’红瑞木炭疽病的病原菌为暹罗炭疽菌和胶孢炭疽菌,这是国内关于红瑞木炭疽病的首次报道。The anthracnose disease of Cornus alba‘Bud’s Yellow’is widespread in Shanghai.In this study,two different types of strains were isolated from infected C.alba‘Bud’s Yellow’leaves collected from Shanghai Disney with anthracnose symptoms,which were confirmed as pathogenic agents by infection assay.Based on multi-locus sequences of ITS(internal transcribed spaces),GAPDH(glyceral-dehyde-3-phosphate dehydrogenase),CAL(calmodulin)and TUB2(β-tubulin 2),concatenated phylogenetic trees were generated by maximum likelihood and Bayesian inference.Two phylogenetic trees showed consistent results in which the pathogenic strains were grouped in the clade as Colletotrichum siamense and C.gloeosporioides.Together with their morphological characteristics,the pathogenic agents were identified as C.siamense and C.gloeosporioides.This is the first report of Colletotrichum species causing anthracnose disease of C.alba in China.

关 键 词：红瑞木 暹罗炭疽菌 胶孢炭疽菌 多基因 致病性 

分 类 号：S432.1[农业科学—植物病理学] S436.85[农业科学—农业昆虫与害虫防治]