整合素β1在大鼠两种脑损伤模型中的差异性表达  

Differential Expression of Integrinβ1 in Two Brain Injury Models of Rats

作　　者：吴茂旺 苏莉 何芳 徐祥 WU Mao-wang;SU Li;HE Fang;XU Xiang(School of Forensic Medicine,Wannan Medical College,Wuhu 241002,Anhui Province,China)

机构地区：[1]皖南医学院法医学院,安徽芜湖241002

出　　处：《法医学杂志》2020年第4期502-506,共5页Journal of Forensic Medicine

基　　金：安徽省教育厅自然科学基金资助项目(KJ2019A0410);国家自然科学基金资助项目(31271155)。

摘　　要：目的研究整合素β1(integrinβ1)在两种创伤性脑损伤模型大鼠脑组织中的阳性表达特征,探讨运用integrinβ1阳性表达推断颅脑损伤致伤方式的可行性。方法采用液压冲击法和单摆式打击法打击大鼠枕部,分别建立直线加速与旋转加速两种闭合性脑损伤模型。120只SD大鼠被随机分为直线加速损伤组、旋转加速损伤组、假手术组及正常对照组。用免疫组织化学染色及Western印迹法检测大鼠伤后30min、3h、6h、12h、3d、7d不同部位脑组织的integrinβ1阳性表达,所得数据经SPSS 18.0软件统计分析。结果脑损伤后30min即可检测到integrinβ1阳性表达,伤后6h达到高峰,随着损伤经过时间的延长,表达呈增强趋势。在伤后相同时间点,直线加速损伤组与旋转加速损伤组integrinβ1在枕部打击点及丘脑部位的阳性表达差异无统计学意义(P>0.05),而在额叶、脑干处表达差异具有统计学意义(P<0.05)。结论Integrinβ1在脑组织内阳性表达的特征可以用来推断打击点及致伤方式,对颅脑损伤经过的重建具有应用价值。Objective To study the characteristics of positive expression of integrinβ1 in the rat brain tissue of two kinds of traumatic brain injury models and to explore the feasibility of inferring the mode of traumatic brain injury using the positive expression of integrinβ1.Methods The occipital region of rats was hit by hydraulic impact method and pendulum striking method to produce two closed brain injury models of linear and rotation acceleration respectively,then 120 SD rats were randomly divided into linear acceleration injury group,rotation acceleration injury group,sham operation group and normal control group.Immunohistochemistry staining and Western blotting method were used to detect the positive expression of integrinβ1 in different parts of the brain tissue at 30 min,3 h,6 h,12 h,3 d and 7 d after rat injury.The data was processed statistically by SPSS 18.0 software.Results The positive expression of integrinβ1 was detected 30 min after brain injury and reached the peak 6 h after brain injury.With the extension of injury time,the expression tended to enhance.At the same time points after injury,the differences in the positive expression of integrinβ1 between the linear acceleration injury group and the rotation acceleration injury group in the occipital strike point and thalamus had no statistical significance(P>0.05),but the differences in the expression of integrinβ1 in the frontal lobe and brain stem had statistical significance(P<0.05).Conclusion The characteristics of positive expression of integrinβ1 in brain tissue can be used to infer the strike point and the manner of injury and has application value for the reconstruction of craniocerebral injury process.

关 键 词：法医病理学 脑损伤 整合素Β1 免疫组织化学 印迹法 蛋白质 致伤方式 

分 类 号：DF795.1[医药卫生—法医学]