陆地棉蔗糖转运蛋白基因家族的鉴定及表达分析  

Identification and Expression Analysis of Sucrose Transporter Gene Family in Upland Cotton(Gossypium hirsutum L.)

作　　者：晁毛妮[1] 王斌[1] 陈煜[2] 张金宝[1] 孙新凯 王清连[1] CHAO Maoni;WANG Bin;CHEN Yu;ZHANG Jinbao;SUN Xinkai;WANG Qinglian(Henan Institute of Science and Technology/Henan Collaborative Innovation Center of Modern Biological Breeding/Henan Key Laboratory for Molecular Ecology and Germplasm Innovation of Cotton and Wheat,Xinxiang,Henan 453003,China;Cotton Research Center of Shandong Academy of Agricultural Sciences,Jinan 250100,China)

机构地区：[1]河南科技学院/现代生物育种河南省协同创新中心/河南省棉麦分子生态与种质创新重点实验室,河南新乡453003 [2]山东棉花研究中心,济南250100

出　　处：《西北植物学报》2020年第8期1303-1312,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31601347);河南省科技攻关计划(202102110014);河南省博士后基金(1902042);十三五国家重点研发计划(2016YFD0101413)。

摘　　要：蔗糖转运蛋白(SUT)在蔗糖从“源”到“库”的运输与分配过程中发挥着重要作用。该研究基于最新公布的陆地棉基因组数据,利用生物信息学和荧光定量PCR等方法,对陆地棉SUT基因家族进行全基因组鉴定,并对他们的表达特性进行系统分析。结果显示:(1)在陆地棉基因组中,共鉴定到18个GhSUT基因(GhSUT1-GhSUT18),他们不均匀地分布在陆地棉11条染色体上。(2)GhSUT蛋白间序列一致性很高,均具有11~12个跨膜结构域,且都定位于质膜。(3)进化关系分析表明,陆地棉GhSUT蛋白主要分布在双子叶植物特有的SUT1亚组,以及单、双子叶植物共有的SUT2亚组和SUT4亚组,其中SUT1亚组成员最多,包含8个GhSUT基因。(4)位于同一亚组的GhSUT基因具有相似的内含子-外显子分布模式,不同亚组间GhSUT基因内含子/外显子数目差异很大。(5)转录组分析表明,GhSUT基因在表达水平上存在差异,GhSUT1和GhSUT10在被检测的组织不表达,GhSUT5、GhSUT14、GhSUT7和GhSUT16在被检测的组织表达量较低,其他GhSUT基因在被检测的组织具有较高的表达水平;另外,GhSUT基因的表达具有组织特异性,其中GhSUT2和GhSUT11主要在“源”和“库”器官中表达,GhSUT6和GhSUT15主要在“库”器官中表达,而GhSUT9和GhSUT18主要在纤维中表达。(6)荧光定量PCR分析表明,GhSUT2在“源”和“库”器官中均具有较高的表达水平,GhSUT6主要在“库”器官包括根、花瓣、纤维和茎中表达,在“源”器官(叶片)中表达量很低;GhSUT18主要在纤维中特异性高表达,在其他组织表达量很低。研究表明,实验验证结果与转录组分析结果相对一致。该研究结果为进一步研究SUT家族基因的功能提供了重要的基因信息,并为棉花产量的提高和纤维品质的改良奠定了理论依据。Sucrose transporter(SUT)plays an important role in the transportation and distribution of sucrose from source to sink.In this study,based on the latest published genomic data of upland cotton,we carried out the complete genome identification of SUT gene family,and systematically analyzed their expression characteristics by bioinformatics and qRT-PCR method in upland cotton.Our results showed that:(1)a total of eighteen GhSUT genes(GhSUT1-GhSUT18)were identified,and were unevenly distributed on 11 chromosomes in upland cotton.(2)The sequence identity of GhSUT proteins was very high,and all of them had 11-12 membrane-spanning domains,and were located on the plasma membrane.(3)Phylogenetic analysis indicated that the GhSUT protein was mainly distributed in the dicots-specific SUT1 subgroup,and SUT2 and SUT4 subgroup which contained both dicotyledon and monocotyledon plants.Among them,SUT1 subgroup had the most members,including 8 GhSUT genes.(4)GhSUT genes within the same subgroup demonstrated similar intron-exon organization.However,the number of intron/exon varied greatly among different subgroups.(5)Transcriptome analysis showed that the expression level of GhSUT genes was different.GhSUT1 and GhSUT10 could not be detected in the tested tissues.The expression level of GhSUT5,GhSUT14,GhSUT7 and GhSUT16 was low in the tested tissues,and other GhSUT genes had high expression level in the tested tissues.In addition,GhSUT gene had tissue-specific expression pattern,in which GhSUT2 and GhSUT11 were mainly expressed in source and sink organs,GhSUT6 and GhSUT15 were mainly expressed in sink organs,while GhSUT9 and GhSUT18 were mainly highly expressed in fibers.(6)Further qRT-PCR analysis showed that GhSUT2 was highly expressed in the source and sink organs.GhSUT6 was mainly expressed in the roots,petals,fibers and stems of sink organs,but very low in the source organs(leaves).GhSUT18 was highly expressed in the fibers and very low in other tissues.The results showed that the experimental results were consistent with thos

关 键 词：蔗糖转运蛋白(SUT) 基因家族 进化分析 组织表达 陆地棉 

分 类 号：Q786[生物学—分子生物学] Q789