细胞游离DNA监测在器官移植中的应用前景  

Application prospect of cell-free DNA monitoring in organ transplantation

作　　者：钱佳俊 郭宏骞 Qian Jiajun;Guo Hongqian(Urology Surgery,Nanjing Drum Tower Hospital,Nanjing 210008,China)

机构地区：[1]南京鼓楼医院泌尿外科,210008

出　　处：《中华移植杂志（电子版）》2020年第4期258-262,共5页Chinese Journal of Transplantation(Electronic Edition)

基　　金：江苏省“科教兴卫工程”医学重点学科(实验室)建设计划(ZDXKB2016014)。

摘　　要：器官移植术后移植物排斥反应与损伤的监测一直未能得到很好的解决。细胞游离DNA(cfDNA)是一种可以在血液、尿液等体液中检测到的片段化、降解DNA。移植术后发生排斥反应时体液中cfDNA特异性升高,甚至在穿刺活检病理确诊排斥反应前数周,该指标已显著升高。有研究表明,供者来源细胞游离DNA(dd-cfDNA)水平与移植物损伤的严重程度和敏感度成正比,可能会成为移植术后检测移植物损伤或急性排斥反应的无创性动态指标。本文对cfDNA在器官移植监测中的研究进展作一综述。Postoperative monitoring of allograft rejection and injury in organ transplant recipients has not been effectively resolved.Cell-free DNA(cfDNA)is a fragmented and degraded DNA that can be detected in blood,urine and other body fluids.Its content in body fluids increases specifically when rejection occures or even a few weeks before the pathological diagnosis of rejection by biopsy.Donor-derived cell-free DNA(dd-cfDNA)may become a noninvasive and dynamic indicator for detecting allograft damage or acute rejection after surgery,because dd-cfDNA levels are proportional to the severity and sensitivity of injury.Studies have shown that dd-cfDNA is proportional to the severity and sensitivity of the injury.This article will review the progress of cfDNA monitoring in organ transplantation.

关 键 词：细胞游离DNA 器官移植 早期监测 动态指标 

分 类 号：R617[医药卫生—外科学]