钾肥对水稻籽粒碳氮代谢相关酶基因表达的影响  

Effect of potassium fertilizer on gene expression of enzymes related to carbon-nitrogen metabolism in rice grains

作　　者：金正勋[1] 王珊 王思宇 王剑 张忠臣[1] 李钢夑 朴钟泽[3] JIN Zhengxun;WANG Shan;WANG Siyu;WANG Jian;ZHANG Zhongchen;LEE Gangseob;PIAO Zhongze(School of Agriculture,Northeast Agricultural University,Harbin 150030,China;Rural Development Administration National Instritute of Agriculture Sciences,JeonjuJeollabuk-do 54874,Korea;Crop Breeding and Cultivation Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)

机构地区：[1]东北农业大学农学院,哈尔滨150030 [2]韩国农村振兴厅农业科学院,全罗北道全州54874 [3]上海市农业科学院作物育种栽培研究所,上海201403

出　　处：《东北农业大学学报》2020年第9期9-17,共9页Journal of Northeast Agricultural University

基　　金：“十三五”国家重点研发计划项目(2017YFD0300506-5);国家科技重大专项重点研发项目省级资金资助(GX18B029);中韩国际合作项目(PJ013647)。

摘　　要：盆栽条件下研究不同钾素施入量对不同穗型水稻品种灌浆成熟期籽粒碳氮代谢相关酶基因转录表达量的影响,旨在为阐明钾素营养对水稻淀粉品质形成分子调控机理提供理论依据。结果表明,灌浆过程中籽粒碳氮代谢相关酶基因GBSS1、AGPL2、SSSⅠ、SBEⅡb、ISA1和GS1:3转录表达量呈先升后降趋势,转录因子OsRSR1基因转录表达量呈先降后升趋势;随钾素施入量增加,灌浆期间籽粒GBSS1基因转录表达量逐渐下调,对其衰减率影响小;籽粒AGPL2、SSSⅠ、SBEⅡb、ISAⅠ基因转录表达量逐渐上调,而AGPL2衰减率先升后降,SSSⅠ衰减率降低,SBEⅡb衰减率升高,对ISAⅠ衰减率无明显影响;使籽粒转录因子RSR1基因转录表达量逐渐下调,而上升率升高;使籽粒GS1:3基因转录表达量逐渐上调,衰减率先升后降。基因转录表达量调控是外源钾素对生物性状调控作用分子基础,外源钾素营养对稻米蒸煮食味品质影响通过籽粒碳氮代谢相关酶基因转录表达量调控实现。Two different types of rice varieties were used to investigate the effects of different soil potassium concentrations on gene expression related to carbon-nitrogen metabolism in rice grains at filling stage,to clarify the molecular mechanism of potassium nutrition on the formation of rice starch quality.The results showed that during the grain filling process,the expression level of carbon-nitrogen metabolism genes,GBSSⅠ,AGPL2,SSSⅠ,SBEⅡb,ISAⅠand GS1:3,increased firstly,and then decreased, while OsRSR1 gene expression exhibited the opposite trend. The increase of soil potassiumconcentration inhibits GBSS1 gene expression at filling stage with few effects on its attenuation rate,promotesthe expression of AGPL2, SSS Ⅰ, SBE Ⅱb and ISA1. However, with respect of the attenuationrate, AGPL2 increased first and then decreased, SSS Ⅰ decreased, SBE Ⅱ b increased, ISA1 had nosignificant changes. In addition, the OsRSR1 expression was gradually down-regulated, while the risingrate increased, but the GS1:3 expression level was gradually up- regulated, and the attenuation ratefirst increased and then decreased. Taken together, the gene transcription and expression was themolecular basis for the regulation of exogenous potassium on biological traits. The effects of exogenouspotassium nutrition on the cooking and eating quality of rice could be achieved via regulation of thetranscription and expression of genes related to grain carbon-nitrogen metabolism.

关 键 词：水稻 钾肥 灌浆成熟期 碳氮代谢相关酶 基因表达 

分 类 号：S511[农业科学—作物学]