基因工程方法增加微藻脂质积累研究进展  

Advances in genetic engineering methods to increase lipid accumulation in microalgae

作　　者：李俊磊 张红兵[1] LI Jun-lei;ZHANG Hong-bing(College of Biological Science and Engineering,Hebei University of Economic and Business,Shijiazhuang 050061,China)

机构地区：[1]河北经贸大学生物科学与工程学院,河北石家庄050061

出　　处：《应用化工》2020年第9期2385-2387,2392,共4页Applied Chemical Industry

基　　金：河北省科技厅重点研发项目(18273612D);河北省高等学校科学技术研究项目(ZD2017230);石家庄市科学技术研究与发展计划项目(191240243A);河北经贸大学校内科研项目(2015KYZ01)。

摘　　要：综述了微藻油脂合成途径的国内外研究现状,介绍了脂肪酸合成途径和Kennedy途径这两个TAG合成的关键步骤,阐述了通过基因工程方法强化脂质合成的成功经验,根据所调控的关键酶,分为增强脂肪酸合成途径、增强Kennedy途径和抑制脂肪酸合成的竞争途径三种调控策略,在此基础上,对未来微藻脂质的研究进行了展望。This paper summarizes the research status of oil synthesis pathway in microalgae at home and abroad,introduces the two key steps of TAG synthesis,fatty acid synthesis pathway and Kennedy pathway,and expounds the successful experience of strengthening lipid synthesis by genetic engineering.According to the key enzymes regulated,they are divided into three regulation strategies:enhancing fatty acid synthesis approach,enhancing Kennedy pathway and inhibiting fatty acid synthesis competition approach.And the future research of microalgal lipids was prospected.

关 键 词：微藻 生物柴油 TAG 脂肪酸合成途径 Kennedy途径 

分 类 号：TQ641[化学工程—精细化工] Q939.99[生物学—微生物学]