10种复方中草药对鲟源海豚链球菌的体外抑菌效果研究  

The antibacterial effects of compound Chinese herbal medicine against Streptococcus iniae from hybrid sturgeon

作　　者：袁国尚 张安青 姚丽 何柯微[1,2] 姜海波 文明[1,4,5] 邵俭 林艳红[1,2,4] Yuan Guoshang;Zhang Anqing;Yao Li;He Kewei;Jiang Haibo;Wen Ming;Shao Jian;Lin Yanhong

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州大学特种水产研究所,贵州贵阳550025 [3]铜仁市农业农村局渔业技术推广站,贵州铜仁554300 [4]贵州省动物疫病与兽医公共卫生重点实验室,贵州贵阳550025 [5]贵州省动物生物制品工程技术研究中心,贵州贵阳550025

出　　处：《饲料工业》2020年第18期13-16,共4页Feed Industry

基　　金：贵州大学大学生“SRT计划”项目[贵大SRT字2018180,贵大SRT字2018187];贵州大学“本科教学工程”建设项目[JG201711];贵州省科技支撑计划项目(黔科合支撑[2016]2502号,[2016]2511号,[2017]2532号);贵州省科技平台及人才团队计划(黔科合平台人才(2018)5253号)。

摘　　要：为筛选对鲟源海豚链球菌(Streptococcus iniae)有较好抑菌作用的复方中草药。采用牛津杯对九味羌活汤、桑菊饮等10种具有抑菌效果的复方中草药进行海豚链球菌体外抑菌试验,并通过二倍稀释法测定了10种复方中草药对海豚链球菌的最小抑菌质量浓度(MIC)和最小杀菌质量浓度(MBC)。结果表明:鲟源海豚链球对九味羌活汤和桑菊饮高度敏感,抑菌圈直径分别为(24.08±3.92)、(23.92±2.34)mm,其MIC值均为62.5 mg/ml,MBC值均为125 mg/ml。鲟源海豚链球菌对养阴清肺汤、八正散、五苓散和大青龙汤4种复方中草药中度敏感,而对槐花散、藿香正气散、麻黄杏仁甘草石膏汤和痰饮丸4种复方中草药低度敏感。九味羌活汤和桑菊饮对鲟源海豚链球菌具有较好的抑菌、杀菌效果。In this study,the antibacterial effects of 10 kinds of compound Chinese herbal medicines were detected,and the minimal inhibitory concentration(MIC)and the minimum bactericidal concentration(MBC)of compound Chinese herbs were measured by microdilution procedure.The results showed that jiuwei qianghuo tang and sangju yin could effectively inhibit the growth of S.iniae with the diameter of inhibition zone of(24.08±3.92)mm and(23.92±2.34)mm.The MIC of jiuwei qianghuo tang and sangju yin were 62.5 mg/ml.The MBC of jiuwei qianghuo tang and sangju yin were 125 mg/ml.The MIC and the MBC of the other compound Chinese herbal medicines against the pathogenic bacteria were higher than 62.5 mg/ml and 125 mg/ml.In conclusion,jiuwei qianghuo tang and sangju yin could use as antimicrobial alternatives to control the S.iniae.

关 键 词：杂交鲟 复方中草药 海豚链球菌 抑菌效果 最小抑菌质量浓度 最小杀菌质量浓度 

分 类 号：S816.3[农业科学—饲料科学]