基于金纳米颗粒聚集的高灵敏Ag^+电致化学发光生物传感器  

Highly Sensitive Ag^+ECL Biosensor Based on Gold Nanoparticles Aggregation

作　　者：苗向阳 王孝英[1] 朱钦舒 Miao Xiangyang;Wang Xiaoying;Zhu Qinshu(Analytical and Testing Center,Nanjing Normal University,Nanjing 210023,China;Department of Medical Science and Technology,Suzhou Chien-shiung Institute of Technology,Taicang 215411,China)

机构地区：[1]南京师范大学分析测试中心,江苏南京210023 [2]苏州健雄职业技术学院医药科技学院,江苏太仓215411

出　　处：《南京师大学报（自然科学版）》2020年第3期63-70,共8页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(21974070);太仓市基础研究计划项目(2018JC04).

摘　　要：银离子是一种环境污染物,对其进行灵敏的检测非常重要.本文构建了一种基于金纳米颗粒(AuNPs)聚集的高灵敏Ag^+电致化学发光(ECL)生物传感器.探针DNA依靠Au-S键连接到金电极(GE)表面,用6-巯基-1-己醇(6-Hydroxy-1-hexanethiol,6-MCH)封闭未结合位点,合成尺寸均一的Au NPs,将其与Link DNA连接,通过与探针DNA杂交连接到电极表面.在杂交过程中加入一定浓度的Ag^+孵育后,由于C-Ag^+-C结构形成,诱导Au NPs大量聚集,提高电子传输效率.最佳条件下,传感器检测Ag^+浓度线性范围为0.5 nmol/L^10μmol/L和10μmol/L^500μmol/L,最低检出限0.25 nmol/L,显示出较好的特异性和稳定性.Silver ion is an environmental pollutant.It is very important to detect it sensitively.In this paper,a highly sensitive Ag^+ECL biosensor based on gold nanoparticle aggregation is constructed.First,the probe DNA is connected to the surface of the gold electrode(GE)by Au-S bond,and the unbound site is blocked by MCH.Subsequently,size-uniform Au NPs are synthesized and connected to the link DNA and then tied to the electrode surface by hybridization with probe DNA.When a certain concentration of Ag^+is added during the hybridization process,the stable C-Ag^+-C structure is formed,which leads to aggregate Au NPs and accelerate the electron transport performance of the sensor.Under optimal conditions,the sensor is used as detector for the concentrations of Ag^+.The linear ranges are obtained in the range of 0.5 nmol/L-10μmol/L and 10μmol/L-500μmol/L.The detection limit of the ECL DNA biosensor is 0.25 nmol/L.This biosensor has good specificity and stability.

关 键 词：C-Ag^+-C 金纳米颗粒 ECL AG^+ 

分 类 号：O657.1[理学—分析化学] TP212.3[理学—化学]