A signal-amplified whole-cell biosensor for sensitive detection of Hg^2+ based on Hg^2+-enhanced reporter module  

作　　者：Dan Wang Yanan Zheng Liudan Wei Na Wei Xiaosu Fan Shan Huang Qi Xiao 

机构地区：[1]College of Chemistry and Materials,Guangxi Key Laboratory of Natural Polymer Chemistry and Physics,Nanring Normal University,Nanning 530001,China [2]Experimental Center of College of Agriculture,Guangxi University,Nanning 530005,China

出　　处：《Journal of Environmental Sciences》2020年第10期93-98,共6页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No.31800631);the Natural Science Foundation of Guangxi Province (No.2018JJB120049);the Middleaged and Young Teachers’ Basic Ability Promotion Project of Guangxi (No.2018KY0361);the BAGUI Scholar Program of Guangxi Province of China。

摘　　要：A signal-amplified mercury sensing biosensor with desired sensitivity was developed through firstly using the GFP mutant with fluorescence increasing response towards Hg^2+ as the reporter module.The developed biosensor showed response for Hg^2+ in a relatively wide range of 1–10,000 nmol/L,and the detection limit was improved one or two orders of magnitude in comparison with most metal-sensing biosensors in similar constructs.In addition,the biosensor could distinguish Hg^2+ easily from multiple metal ions and displayed strong adaptability to extensive p H conditions (pH 4.0–10.0).More importantly,the developed biosensor was able to provide an initial assessment of Hg^2+ spiked in the environmental water with the recoveries between 85.70%and 112.50%.The signal-amplified strategy performed by the modified reporter module will be widely applicable to many other wholecell biosensors,meeting the practical requirements with sufficient sensing performance.

关 键 词：Mercury sensing biosensor Signal-amplified Reporter module Environmental samples monitoring 

分 类 号：X85[环境科学与工程—环境工程] TP212.3[自动化与计算机技术—检测技术与自动化装置]