Dye reduction-based electron-transfer activity monitoring assay for assessing microbial electron transfer activity of microbial fuel cell inocula  

作　　者：Kartik S.Aiyer Roshan Rai B.S.Vijayakumar 

机构地区：[1]Department of Biosciences,Sri Sathya Sai Institute of Higher Learning,Prasanthi Nilayam,Puttaparthi,Andhra Pradesh-515134,India

出　　处：《Journal of Environmental Sciences》2020年第10期171-177,共7页环境科学学报（英文版）

摘　　要：Microbial fuel cells (MFC) utilize microbes as catalysts to convert chemical energy to electricity.Inocula used for MFC operation must therefore contain active microbial population.The dye reduction-based electron-transfer activity monitoring (DREAM) assay was employed to evaluate different inocula used in MFCs for their microbial bioelectrical activity.The assay utilizes the redox property of Methylene Blue to undergo color change from blue to colorless state upon microbial reduction.The extent of Methylene Blue reduction was denoted as the DREAM assay coefficient.DREAM assay was initially performed on a microbial culture along with the growth curve and estimation of colony forming units (CFUs).DREAM coefficient correlated to the CFU/m L obtained over time as growth progressed.The assay was then extended to water samples (domestic sewage,lake and a man-made pond) serving as inocula in MFCs.Domestic wastewater gave the highest DREAM coefficient (0.300±0.05),followed by pond (0.224±0.07) and lake (0.157±0.04) water samples.Power density obtained conformed to the DREAM coefficient values,with the three samples generating power densities of 46.45±5.1,36.12±3.2 and 25.08±4.3 m W/m^2 respectively.We have also studied the role of addition of various carbon sources and their concentrations towards improving the sensitivity of the assay.The DREAM assay is a rapid,easy-to-perform and cost-effective method to assess inocula for their suitability as anolytes in terms of electron transfer potential in MFCs.

关 键 词：Dye reduction Electron transfer Methylene Blue Microbial fuel cell Wastewater 

分 类 号：X703[环境科学与工程—环境工程] TM911.45[电气工程—电力电子与电力传动]