A Simplified Continuous Two-stitch Suture for Bronchial Anastomosis of Left Single Lung Transplant in Dogs  

作　　者：Ping LI Lan ZHU Fei-fei TANG Jing XIONG Ming-jia MA Mouniir DSA Si-hai GAO 

机构地区：[1]Department of Cardiothoracic and Vascular Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430030,China [2]Key Laboratory of Organ Transplantation,Chinese Ministry of Education and Chinese Ministry of Health,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430030,China [3]Department of Cardiovascular Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430022,China [4]Institute of Pathology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430030,China

出　　处：《Current Medical Science》2020年第3期548-555,共8页当代医学科学（英文）

基　　金：This work was supported by a grant from the National Natural Science Foundation of China(No.81570090).

摘　　要：Large animal models are essential to pre-clinical trials of pulmonary transplantation and bronchial anastomosis poses a great technical challenge to the procedure.Presented here is a simplified continuous two-stitch suture technique into bronchial anastomosis during the course of left single lung transplantation in canine.Animals were divided into three groups with each group having 6 animals.Left single lung transplantation in canine was performed to assess the feasibility of using this technique for bronchial anastomosis.In the control groups,all anastomoses were done by using traditional technique.Allograft functions and hemodynamic parameters were monitored during a 3-h reperfusion period.Quality of bronchial healing and airway complications were assessed by bronchoscopic surveillance after transplantation.We successfully completed left lung transplantation in 18 dogs,and all the dogs survived the procedures.The new technique substantially simplified the procedures for bronchial anastomosis and greatly reduced the time for bronchial anastomosis(P<<0.01)and the ischemic time of the grafts(P<0.05)compared to the control group.The continuous two-stitch suture attenuated the tissue injury to allografts and led to better blood gas exchange function as compared to the control group(P<0.05).Good bronchial healing(Grade I)was observed in all the groups.A canine left single lung transplantation model is feasible by using the novel suture technique,and the new technique is as safe as the traditional method.The technique is easy to learn,particularly for less experienced operators.Simpler and time-saving,the technique has great potential to be widely employed in clinical lung transplantation.

关 键 词：lung transplantation bronchial anastomosis animal model 

分 类 号：R56[医药卫生—呼吸系统]