基于液质联用的枳实薤白桂枝汤化学成分研究  

Investigation of Chemical Constituents and Sources of Zhishi Xiebai Guizhi Decoction by LC-MS/MS

作　　者：曲珍妮 齐欣[2] 谭培艺[3] 段宏泉[1] 董林毅[1] QU Zhen-ni;QI Xin;TAN Pei-yi;DUAN Hong-quan;DONG Lin-yi(College of Pharmacy,Tianjin Medical University,Tianjin 300070,China;Zhongxin Pharma-Tianjin No.6 TCM Factory,Tianjin 300401,China;Department of Pharmacy,Tianjin Chinese Medical University 1st Hospital,Tianjin 300381,China)

机构地区：[1]天津医科大学药学院,天津300070 [2]天津中新药业集团股份有限公司第六中药厂,天津300401 [3]天津中医药大学第一附属医院药学部,天津300381

出　　处：《中国药学杂志》2020年第17期1421-1424,共4页Chinese Pharmaceutical Journal

基　　金：天津中医药大学第一附属医院“拓新工程”科研项目资助(201902);天津市科委项目资助(17JCQNJC13300)。

摘　　要：目的通过高效液相-质谱联用的方法,研究枳实薤白桂枝汤及其单味药的化学成分。对枳实薤白桂枝汤中化学成分进行初步分析及色谱峰归属,为科学评价和质量控制提供科学依据。方法采用Agilent XDB-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-水为流动相,梯度洗脱,对枳实薤白桂枝汤全方、单味药进行HPLC-IT/MS分析,在正负离子模式下采集数据,结合文献报道,对枳实薤白桂枝汤水煎液主要化学成分进行分析鉴定。结果从枳实薤白桂枝汤中初步鉴定19个化合物,主要成分为柚皮苷、新橙皮苷等,主要成分类型包括生物碱类、甾体皂苷类、糖苷类及黄酮类等。结论本实验比较全面的阐明了枳实薤白桂枝汤的化学组成,对枳实薤白桂枝汤的鉴别和质量评价有重要的参考价值。OBJECTIVE To study the chemical constituents of Zhishi Xiebai Guizhi decoction and its single agent by high performance liquid chromatography-mass spectrometry to provide a scientific basis for the quality evaluation and control.METHODS Agilent XDB-C18(4.6 mm×250 mm,5μm)column was used for HPLC analysis with acetonitrile-water as mobile phase by gradient elution.Using liquid chromatography-mass spectrometry technology to collect data in positive and negative ion mode,combined with literature reports,the main chemical components of Zhishi Xiebai Guizhi decoction were analyzed and identified.RESULTS A total of 19 compounds were identified from Zhishi Xiebai Guizhi decoction.The main components are naringin,neohesperidin,etc.The main component types include alkaloids,steroidal saponins,glycosides,flavonoids,etc.CONCLUSION This study comprehensively clarified the chemical composition of Zhishi Xiebai Guizhi decoction,and it has important reference value for the identification and quality evaluation of Zhishi Xiebai Guizhi decoction.

关 键 词：枳实薤白桂枝汤 高效液相-质谱联用 色谱峰归属 柚皮苷 新橙皮苷 

分 类 号：R284[医药卫生—中药学]