肝素抗凝血浆用于常规生化项目检测的可行性研究  

Feasibility study on the use of heparin anticoagulant plasma for the detection of routine biochemical tests

作　　者：谭延国[1] 肖阳 亢涛[1] 曾海青[1] 王志阳[1] 张磊[1] 张莉[1] 李佩[1] 李格[1] 钟侃 张岩[1] TAN Yanguo;XIAO Yang;KANG Tao;ZENG Haiqing;WANG Zhiyang;ZHANG Lei;ZHANG Li;LI Pe;LI Ge;ZHONG Kan;ZHANG Yan(Department of Clinical Laboratory,Fu Xing Hospital,Capital Medical University,Beijing 100038,China;Department of Laboratory Medicine,Capital Medical University,Beijing 100038,China)

机构地区：[1]首都医科大学附属复兴医院检验科,北京100038 [2]首都医科大学医学检验系,北京100038

出　　处：《国际检验医学杂志》2020年第19期2316-2319,共4页International Journal of Laboratory Medicine

摘　　要：目的探讨肝素锂抗凝的血浆能否代替血清用于常规生化项目的测定。方法选取在该院检测常规生化检验项目的40例患者,每位患者同时采集血清标本与肝素锂抗凝血浆标本各一份,分别使用同一台全自动生化分析仪测定常规生化项目39项。可接受标准(须同时符合以下条件):(1)相关系数(r)≥0.975;(2)回归方程的斜率在0.95~1.05;(3)对同一指标,90%以上患者血浆相对血清结果的偏倚≤1/2允许总误差(TEa)。结果39项生化检测指标中,符合判定标准的有总胆红素(TBIL)、清蛋白(ALB)、尿酸(UA)、载脂蛋白B100(APOB100)、肌酸激酶(CK)、同型半胱氨酸(HCY)、血糖(GLU)、总胆汁酸(TBA)、前清蛋白(PA)、高密度脂蛋白(HDL)、载脂蛋白A1(APOA1)、脂蛋白(LPa)和碱性磷酸酶(ALP)共13项。结论大多数临床常规生化项目血浆与血清结果不符,在考虑使用血浆替代血清时应特别审慎。Objective To investigate whether heparin lithium anticoagulant plasma samples could replace serum in the determination of routine biochemistry tests.Methods A total of 40 patients were selected for routine biochemical test in a hospital.The serum specimen and anticoagulant plasma specimen of heparin lithium were collected from each patient,respectively.Thirty-nine routine biochemistry tests were measured by the same automatic biochemical analyzer.Acceptable criteria(must meet the following conditions at the same time):(1)correlation coefficient(r)≥0.975;(2)the slope coefficient of the regression equation is 0.95-1.05;(3)for the same indicator,the plasma bias relative to serum results in more than 90%patients was less than or equal to half TEa,which was the maximum permissible errors according to CLIA′88 criteria.Results Only 13(TBIL,ALB,UA,APOB100,CK,HCY,GLU,TBA,PA,HDL,APOA1,LPa and ALP)out of the 39 items met the criteria.Conclusion Plasma and serum results do not match in most routine biochemistry tests,and special care should be taken when considering the use of plasma instead of serum.

关 键 词：肝素锂抗凝血浆 生化检验指标 可行性 允许总误差 

分 类 号：R446.1[医药卫生—诊断学]