有核红细胞计数在不同全自动血细胞分析仪中的应用及评价  

Application and evaluation of nucleated red blood cell count in different automatic hematology analyzers

作　　者：孟欣[1] 王苗苗 王刚[1] 王晓琴[1] 张宁[1] MENG Xin;WANG Miaomiao;WANG Gang;WANG Xiaoqin;ZHANG Ning(Department of Clinical Laboratory,the First Affiliated Hospital of Xi′an Jiaotong University,Xi′an,Shaanxi 710061,China)

机构地区：[1]西安交通大学第一附属医院检验科,陕西西安710061

出　　处：《国际检验医学杂志》2020年第19期2392-2396,共5页International Journal of Laboratory Medicine

摘　　要：目的探讨Sysmex XN-9000和Mindray BC-6800 Plus全自动血细胞分析仪对有核红细胞(NRBC)的检测性能。方法收集该院门诊、住院患者血液标本共计13156份,经推片镜检,确认208份标本NRBC阳性,计算Sysmex XN-9000和Mindray BC-6800 Plus全自动血细胞分析仪检测NRBC的灵敏度、特异度、阳性预测值和阴性预测值。两种仪器与推片镜检NRBC结果相关性和一致性比较分析,比较不同水平NRBC两种仪器WBC仪器校正、人工校正及NRBC各参数结果差异,以及各仪器法与推片镜检NRBC结果差异时WBC仪器校正、人工校正及NRBC各参数差异。结果Sysmex XN-9000全自动血细胞分析仪NRBC计数的灵敏度、特异度、阳性预测值和阴性预测值分别为93.75%、99.70%、83.33%、99.90%,Mindray BC-6800 Plus全自动血细胞分析仪NRBC计数的灵敏度、特异度、阳性预测值和阴性预测值分别为79.81%、99.92%、93.79%、99.68%。Sysmex XN-9000与推片镜检NRBC结果呈正相关(r=0.966,P<0.05)。Mindray BC-6800 Plus与推片镜检NRBC结果呈正相关(r=0.963,P<0.05)。两种仪器的Bland-Altman偏移散点图均显示,随着NRBC均值的增大,散点的分布呈现分散状态。当镜检NRBC<10%,WBC、WBC仪器校正结果、NRBC人工计数结果及仪器间计数结果在两个系统中差异均无统计学意义(P>0.05)。当镜检NRBC>10%,WBC、WBC人工计数结果及仪器间计数结果在两个系统中差异无有统计学意义(P>0.05),而WBC人工校正结果、仪器NRBC计数差异均有统计学意义(P<0.01)。Sysmex XN-9000全自动血细胞分析仪NRBC镜检小于仪器结果与镜检大于仪器结果比较,WBC、WBC仪器校正结果、WBC人工校正结果、NRBC计数差异均有统计学意义(P<0.05)。Mindray BC-6800Plus全自动血细胞分析仪NRBC镜检小于仪器结果与镜检大于仪器结果比较,WBC、WBC仪器校正结果差异有统计学意义(P<0.05),而WBC人工校正结果、NRBC计数差异无统计学意义(P>0.05)。结论当外周血NRBC�Objective To investigate the detection performance of nuclear red cells(NRBC)in Sysmex XN-9000 and Mindray BC-6800 Plus automatic hematology analyzers.Methods A total of 13156 blood samples were collected from outpatient and inpatient patients in our hospital,in which 208 cases were confirmed to be NRBC positive by microscopy.The sensitivity,specificity,positive predictive value and negative predictive value of NRBC were calculated by two hematology analyzers.The NRBC results correlation and consistency between analyzers and microscopy,as well as the differences in WBC automatic correction,manual correction and NRBC at different levels between the analyzers and microscopy.Results The sensitivity,specificity,positive predictive value and negative predictive value of NRBC by Sysmex XN-9000 were 93.75%,99.70%,83.33%and 99.90%,and the sensitivity,specificity,positive predictive value and negative predictive value of NRBC by Mindray BC-6800 Plus were 79.81%,99.92%,93.79%and 99.68%,respectively.Sysmex XN-9000 was positively correlated with the NRBC result of microscopy(r=0.966,P<0.05),and Mindray BC-6800 Plus was positively correlated with the NRBC result of microscopy(r=0.963,P<0.05).Bland-Altman plots show that the distribution of the scatter appears to be dispersed with the increase of the mean value of NRBC.When microscopy of NRBC<10%,there were no statistically significant differences in WBC,WBC automatic correction,NRBC manual correction and inter-instrument counting results between the two systems(P>0.05).When microscopy of NRBC>10%,there were no statistically significant difference in WBC,WBC manual counting results and inter-instrument counting results between the two systems(P>0.05),while there were statistically significant differences between WBC manual correction results and NRBC automatic correction(P<0.01).The differences in NRBC,WBC automatic correction and WBC manual correction were statistically significant between NRBC"microscopy less than automatic correction"and"microscopy more than automatic corre

关 键 词：有核红细胞 血细胞分析仪 镜检 敏感性与特异性 

分 类 号：R446.1[医药卫生—诊断学]