环境雌激素SERS检测的研究进展  

Recent Progress of SERS for Environmental Estrogen Detection

作　　者：刘小红 邓华[1] 常林 张炜[2] 姜珊 LIU Xiao-hong;DENG Hua;CHANG Lin;ZHANG Wei;JIANG Shan(Chongqing Youth Vacational&Technical College,Chongqing 400712,China;Chongqing Institute of Green and Intelligent Technology,Chinese Academy of Sciences,Chongqing 400714,China)

机构地区：[1]重庆青年职业技术学院,重庆400712 [2]中国科学院重庆绿色智能技术研究院,重庆400714

出　　处：《光谱学与光谱分析》2020年第10期3038-3047,共10页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金面上项目(61575196);重庆市自然科学基金面上项目(cstc2019jcyj-msxmX0663);重庆市教委科学技术研究项目(KJQN201904102);北碚区基础研究与前沿探索项目(2019-2)资助。

摘　　要：环境雌激素是一种外源性内分泌干扰物,一般以较低浓度存在于环境中,可在生物体内富集并扰乱内分泌系统的正常功能,诱导生物体发生多种病变,因此对其进行低浓度高特异性的检测具有重要意义。表面增强拉曼散射(SERS)是一种能够进行痕量分析甚至单分子水平检测的技术。文章综述了近几年SERS技术对环境雌激素(主要为雌二醇、多氯联苯、双酚A等)的检测现状。主要从两个方向进行总结分析:首先,不进行修饰直接利用SERS基底进行检测;其次,用(环糊精、分子印迹聚合物、抗原抗体、核酸适配体等)修饰后的基底对环境雌激素进行特异性和高灵敏性检测。最后,对环境雌激素的SERS检测进行了总结与展望。The Environmental estrogen is an exogenous endocrine disruptor which can be enriched in organisms,and usually exits in the environment at a low concentration.Meanwhile,it can interfere with the normal function of the endocrine system and induce the multiple organism pathological changes.Therefore,the detection of environmental estrogen at low concentration with high specificity is important.Surface enhanced Raman scattering(SERS)is a technique with high sensitivity enabling trace amount analysis and even single-molecule level detection of the target molecule.This paper aims to summarize the recent development of environmental estrogen(such as estradiol,polychlorinated biphenyls,bisphenol A,etc.)detected by using SERS technology.Firstly,the SERS substrates without modification are used for direct detection.Secondly,the modified substrates(such as cyclodextrin,molecularly imprinted polymer,antigen and antibody and nucleic acid aptamer,etc.)are used for highly specific and sensitive detection of environmental estrogens.Finally,the development of SERS in environmental estrogen detection are summarized and prospected.

关 键 词：环境雌激素 表面增强拉曼散射 痕量分析 高灵敏性检测 核酸适配体 

分 类 号：O433.4[机械工程—光学工程]