Effect of Dihydrotestosterone on Costimulatory Molecules in a Mouse Model of Graves’ Disease  

作　　者：Lianye Liu Bingyin Shi Fengyi Zhao Peng Hou Shu Liu Xiaomei Liu Liping Wu 

机构地区：[1]Department of Endocrinology,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,China [2]Department of Endocrinology,Weinan Central Hospital,Weinan,Shaanxi 714000,China [3]Department of Endocrinology,Nanjing First Hospital,Nanjing Medical University,Nanjing 210001,China

出　　处：《Chinese Medical Sciences Journal》2020年第3期215-225,共11页中国医学科学杂志（英文版）

基　　金：supported by the National Natural Science Foundation(grant number 81670725,2017.01-2020.12);Key Research and Development Project of Shaanxi Province(grant number 2017ZDXM-SF-060,2017.01-2019.12)。

摘　　要：Objective Graves’disease is the most common autoimmune thyroid disease and its prevalence and clinical manifestations are disparate between females and males.Costimulatory molecules play an essential role in regulating autoimmune responses.The objective of this study was to determine if expression of inhibitory molecules was correlated with treatment by dihydrotestosterone(DHT)in an in vivo BALB/c mouse model of experimental autoimmune Graves’disease.Methods Female BALB/c mice were immunized three times with thyroid stimulating hormone receptor A-subunit encoded by adenovirus to establish a Graves’disease model.Three different doses of DHT or a matching placebo were administered by implantation of slow-release pellets a week before the first immunization.Four weeks after the third immunization,the mice were euthanatized,and then the spleen and thymus were removed.Total thyroxine and free thyroxine levels in serum of mice were detected using a radioimmunoassay kit.Real-time polymerase chain reaction was performed to estimate the expression of costimulatory molecules in lymphocytes from the spleen and thymus.Flow cytometry was used to analyze the percentage of CD4^+T cells in splenic lymphocytes.Quantitative data were compared with unpaired t-tests.Correlation between two variables was analyzed using Analysis of Variance.Results Treatment with DHT can dramatically reduce total thyroxine and free thyroxine levels.Higher expression of programmed death-1 was found in the spleen of Graves’disease mice receiving 5 mg of DHT treatment(0.635±0.296 vs.0.327±0.212;t=2.714,P=0.014),similarly,T-cell immunoglobulin domain and mucin domain 3(TIM-3)in both the spleen(1.004±0.338 vs.0.646±0.314;t=2.205,P=0.022)and the thymus(0.263±0.127 vs.0.120±0.076;t=3.221,P=0.004)also increased after 5 mg of DHT treatment compared with the parallel placebo model mice.Moreover,the percentage of CD4^+T cells declined in the splenic lymphocytes of Graves’disease mice treated with 5 mg of DHT(19.90%±3.985%vs.24.05%±2.587%;t=2.80

关 键 词：Graves’disease 5α-dihydrotestosterone costimulatory molecules inhibitory molecules 

分 类 号：R581.1[医药卫生—内分泌] R-332[医药卫生—内科学]