放置时间对人类白细胞抗原-B27检测结果准确性的影响  

Effect of storage time on accuracy of detection of human leukocyte antigen-B27

作　　者：严海东 王珂[2] 高海珍[2] YAN Haidong;WANG Ke;GAO Haizhen(Department of Clinical Laboratory,Jiangyin City Hospital of Traditional Chinese Medicine,Wuxi,Jiangsu,214440;Department of Clinical Laboratory,Jiangyin Hospital Affiliated to Medical College of Southeast University,Wuxi,Jiangsu,214440)

机构地区：[1]江苏省江阴市中医院检验科,江苏无锡215400 [2]东南大学医学院附属江阴医院检验科,江苏无锡214400

出　　处：《实用临床医药杂志》2020年第15期11-13,共3页Journal of Clinical Medicine in Practice

基　　金：江苏省无锡市卫生计生科研面上项目(MS201642)。

摘　　要：目的探讨放置时间对人类白细胞抗原-B27(HLA-B27)检测结果准确性的影响。方法采用HLA-B27FITC/CD3 PE双色荧光抗体染色法,应用流式细胞术进行样本检测,比较染色后放置时间及血标本放置时间对检测结果的影响。结果染色后放置0、20、40、60、80、100、120、180、300 min时通过荧光1检测通道(FL1)检测出的平均荧光强度值分别为(139.7±27.1)、(136.6±26.2)、(133.2±26.3)、(130.9±25.3)、(128.7±25.4)、(127.3±25.1)、(126.2±24.6)、(122.4±24.0)、(116.6±22.1)。随着放置时间的延长,标本平均荧光强度逐渐下降,且平均荧光强度的衰减与放置时间相关。血样放置0 min及1、2、4、8、24、48 h时平均荧光强度值分别为(139.7±27.1)、(140.0±26.9)、(140.1±27.3)、(139.0±26.9)、(140.3±26.0)、(139.0±27.7)、(135.1±26.4),其中血样放置48 h时结果与新鲜标本(放置0 min)结果比较,差异有统计学意义(P<0.05)。结论标本制备后应立即进行检测,血标本采集后应在24 h内进行检测。Objective To investigate the effect of storage time on the accuracy of detection of human leukocyte antigen-B27(HLA-B27).Methods HLA-B27 FITC/CD3 PE double color fluorescent antibody staining was used,and flow cytometry was used to detect samples.The influence of storage time after staining and blood sample storage time on the detection results was compared.Results The average fluorescence intensity values detected by fluorescence 1 detection channel(FL1)at 0,20,40,60,80,100,120,180 and 300 min after staining were(139.7±27.1),(136.6±26.2),(133.2±26.3),(130.9±25.3),(128.7±25.4),(127.3±25.1),(126.2±24.6),(122.4±24.0)and(116.6±22.1)respectively.With the extension of storage time,the average fluorescence intensity values of the samples decreased gradually,and the attenuation of the average fluorescence intensity was correlated with the storage time.The average fluorescence intensity values of blood samples at 0 min and 1,2,4,8,24 and 48 h were(139.7±27.1),(140.0±26.9),(140.1±27.3),(139.0±26.9),(140.3±26.0),(139.0±27.7)and(135.1±26.4)respectively.The result of blood samples stored for 48 h showed significant difference compared with that of fresh samples placed for 0 min(P<0.05).Conclusion The samples should be detected immediately after preparation,and blood samples should be detected within 24 hours after collection.

关 键 词：人类白细胞抗原-B27 流式细胞术 强直性脊柱炎 放置时间 

分 类 号：R446.11[医药卫生—诊断学]