鼠神经生长因子对三叉神经痛大鼠三叉神经节氧化应激和炎性因子的影响  

Effects of rat nerve growth factor on oxidative stress and inflammatory factors of trigeminal ganglia in rats with trigeminal neuralgia

作　　者：苏鸿年[1] 司马靓杰[2] 杨木强[2] SU Hong-nian;SIMA Liang-jie;YANG Mu-qiang(Department of Pain,The First Affiliated Hospital of Henan University of Science and Technology,Luoyang 471003,Henan Province,China;Department of Anesthesiology,The First Affiliated Hospital of Henan University of Science and Technology,Luoyang 471003,Henan Province,China)

机构地区：[1]河南科技大学第一附属医院疼痛科,河南洛阳471003 [2]河南科技大学第一附属医院麻醉科,河南洛阳471003

出　　处：《中国临床药理学杂志》2020年第17期2664-2667,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探讨鼠神经生长因子对三叉神经痛大鼠三叉神经节氧化应激和炎性因子的影响。方法采用左侧眶下神经(ION-CCI)环扎术建立三叉神经痛大鼠模型;另选大鼠只暴露眶下神经,但不结扎,其余操作一致作为空白组。按照体重将造模成功大鼠分为5组:模型组、阳性对照组和低剂量实验组、中剂量实验组、高剂量实验组,每组15只;术后14 d,低、中、高3个剂量实验组硬膜外腔注射鼠神经生长因子0.25,0.5,1μL·kg^-1,qd,空白组和模型组硬膜外腔注射等量0.9%NaCl,阳性对照组(7.5 mg·kg^-1卡马西平药液,qd)灌胃,给药7 d。用比色法检测谷胱甘肽过氧化物酶(GSH-Px)活性,以硫代巴比妥酸法检测超氧化物歧化酶(SOD)活性,以黄嘌呤氧化法检测丙二醛(MDA)含量。用酶联免疫吸附法检测血清白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL^-1β)等炎性因子水平。结果末次给药1 h后,空白组、模型组、阳性对照组、低、中、高剂量实验组的GSH-Px水平分别为(112.48±11.97),(87.64±9.88),(98.72±11.25),(88.65±10.21),(100.54±10.91)和(111.52±11.88)U·L^-1;SOD水平分别为(228.12±27.61),(169.67±20.99),(213.54±25.43),(172.54±19.82),(189.22±28.01)和(225.46±28.68)U·mL^-1;MDA水平分别为(6.38±1.22),(11.48±2.62),(8.02±1.45),(10.82±1.33),(7.44±1.39)和(6.59±1.19)nmol·mL^-1;IL-6水平分别为(4.02±0.64),(6.18±0.56),(4.86±0.56),(6.07±0.61),(4.87±0.53)和(4.61±0.52)pg·mL^-1;TNF-α水平分别为(77.59±4.59),(113.03±4.84),(83.93±4.26),(110.66±4.41),(83.55±4.31)和(81.08±4.62)pg·mL^-1;IL^-1β水平分别为(9.94±1.62),(14.36±1.73),(11.11±1.58),(14.09±1.53),(11.08±1.54)和(10.86±1.59)pg·mL^-1;模型组与空白组比较、阳性对照组与模型组比较、中剂量实验组与模型组比较或高剂量组与模型组比较,上述指标的差异均有统计学意义(均P<0.05)。结论鼠神经因子可能通过抗氧化应激作用及降低炎症反应以提高疼痛阈�Objective To investigate the effects of rat nerve growth factor on oxidative stress and inflammatory factors of trigeminal ganglia in rats with trigeminal neuralgia.Methods The rat model of trigeminal neuralgia was established by infraorbital nerve chronic constriction injury(ION-CCI)ligation.Rats in blank group were selected to expose only the inferior orbital nerve without ligation,and the rest were treated as model group.Rats successfully modeled were divided into 5 groups according to body weight:model group,positive control group,test-L/M/-H group with 15 in each group.At 14 days after surgery,rats in the test-L/M/-H group were injected into the epidural space with 0.25,0.5,and 1μL·kg^-1 of rat nerve growth factor,qd,the blank group and the model group were given the same amount of 0.9%NaCl,and The positive control group was intragastrically administered with 7.5 mg·kg^-1 carbamazepine solution for 7 days.the activity of serum glutathione peroxidase(GSH-Px)was detected by colorimetric methods,the activity of superoxide dismutase(SOD)was detected by thiobarbital acid method,and the contents of malondialdehyde(MDA)was detected by xanthine oxidation methods.Serum levels of interleukin-6(IL-6),tumor necrosis factor-alpha(TNF-α),interleukin-1 beta(IL^-1β)and other inflammatory factors were determined by ELISA.Results After 1 h of the last administration,the levels of GSH-Px in the blank group,the model group,the positive control group,the test-L/M/-H group were(112.48±11.97),(87.64±9.88),(98.72±11.25),(88.65±10.21),(100.54±10.91)and(111.52±11.88)U·L^-1,respectively.SOD levels in each group were(228.12±27.61),(169.67±20.99),(213.54±25.43),(172.54±19.82),(189.22±28.01)and(225.46±28.68)U·mL^-1,respectively.MDA levels in each group were(6.38±1.22),(11.48±2.62),(8.02±1.45),(10.82±1.33),(7.44±1.39)and(6.59±1.19)nmol·mL^-1,respectively.The levels of IL-6 in the blank group,the model group,the positive control group,the test-L/M/-H group were(4.02±0.64),(6.18±0.56),(4.86±0.56),(6.07±0.61),(4

关 键 词：鼠神经生长因子 三叉神经痛 三叉神经节 氧化应激 炎性因子 

分 类 号：R97[医药卫生—药品]