痤疮发病机制关键基因和通路的生物信息学研究  

Identification of key gene and pathways in the pathogenesis of acne based on bioinformatics analysis

作　　者：秦思[1] 黄锦萍 温炬[1,2] 黄舒婷 李婷 郑荣昌 李华润[1,2] Qin Si;Huang Jinping;Wen Ju;Huang Shuting;Li Ting;Zheng Rongchang;Li Huarun(Department of Dermatology,Guangdong Second Provincial General Hospital,Guangzhou 510317,China;The Second School of Clinical Medicine,Southern Medical University,Guangzhou 510282,China;Department of General Practice,Guangdong Second Provincial General Hospital,Guangzhou 510317,China)

机构地区：[1]广东省第二人民医院皮肤性病科,广州510317 [2]南方医科大学第二临床医学院,广州510282 [3]广东省第二人民医院全科医学科,广州510317

出　　处：《中华医学美学美容杂志》2020年第4期313-317,共5页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：广东省自然科学基金(2015A030313685)。

摘　　要：目的通过生物信息学分析方法探讨痤疮发病机制中可能起重要作用的关键基因和通路。方法从GEO数据库获取GSE6475和GSE53795数据集比较18个痤疮皮损组织和18个正常皮肤组织。用DAVID数据库对差异基因进行基因本体论分析和基因组百科全书关键通路分析,建立差异基因的蛋白互作网络,获取关联性最强的枢纽基因和重要的模块。结果筛选出痤疮组织和正常皮肤组织中314个上调差异基因和62个下调差异基因。KEGG通路分析显示,这些差异基因主要富集在金黄色葡萄球菌感染、破骨细胞分化、戊糖和葡萄糖醛酸酯的相互转化、趋化因子信号途径、药物代谢-其他酶等通路中。建立的PPI网络筛选出379个节点及10个枢纽基因(CXCL8、PTPRC、IL1B、ITGB2、CXCR4、ICAM1、CCR5、SELL、C3AR1、PLEK)。结论枢纽基因和关键通路可能是痤疮发病机制中可进行干预的新靶点。Objective To explore the key genes and pathways that may play an important role in the pathogenesis of acne by bioinformatics analysis.Methods GSE6475 and GSE53795 datasets were collected from GEO database,and 18 acne lesions tissues and 18 normal skin tissues were compared.David database was used to analyze the gene ontology(GO)and the key pathway(Kyoto Encyclopedia of genes and genomes,KEGG)of the differential genes,to establish the protein interaction network of the differential genes,and to obtain the most relevant key genes and important clusters.Results A total of 314 up-regulated genes and 62 down-regulated genes were filtered from those GEO profiles.KEGG pathway analysis showed that these differential genes were mainly enriched in Staphylococcus aureus infection,osteoclast differentiation,pentose and glucuronate interconversions.In addition,379 nodes and ten key genes(CXCL8,PTPRC,IL1B,ITGB2,CXCR4,ICAM1,CCR5,SELL,C3AR1 and PLEK)were screened out by protein interaction network.Conclusions The key genes and pathways identified in this study may be new targets for intervention in the development of acne.

关 键 词：痤疮 生物信息学 差异基因 发病机制 关键通路 

分 类 号：R758.733[医药卫生—皮肤病学与性病学] Q811.4[医药卫生—临床医学]