大黄素对建鲤生长性能、抗氧化指标及肝脏CYP450酶的影响  

Effects of dietary emodin on antioxidant ability,growth and hepatopancreatic CYP450 enzyme activity in common carp Cyprinus carpio Jian

作　　者：曹丽萍 杜金梁[1,2] 贾睿 丁炜东[1,2] Jeney Galina 徐跑[1,2] 殷国俊 CAO Liping;DU Jinliang;JIA Rui;DING Weidong;Jeney Galina;XU Pao;YIN Guojun(Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Wuxi 214081, China;International Joint Research Laboratory for Fish Immunopharmacology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;Research Institute for Fisheries,Aquaculture and Irrigation, Ann light 8, Szarvas H-4440, Hungary)

机构地区：[1]农业农村部淡水渔业和种质资源利用重点实验室,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心农业农村部鱼类免疫药理学国际联合实验室,江苏无锡214081 [3]Research Institute for Fisheries,Aquaculture and Irrigation,Ann light 8,Szarvas H-4440,Hungary

出　　处：《大连海洋大学学报》2020年第5期686-692,共7页Journal of Dalian Ocean University

基　　金：中央级公益性科研院所基本科研业务费专项(2019JBFM11);国家自然科学基金(31702318);江苏省自然科学基金(BK20170218)。

摘　　要：为全面评价大黄素(emodin,EMD)对水产动物的保肝作用,在基础饲料中添加含1、3、5 g/kg的大黄素,将100尾初体质量约150 g的建鲤Cyprinus carpio Jian随机分为5组,分别为空白对照组(control)、模型组(CCl4)、低剂量药物组(1 g/kg EMD)、中剂量药物组(3 g/kg EMD)和高剂量药物组(5 g/kg EMD),每组设2个平行,每个平行10尾鱼,投喂60 d后,一次性腹腔注射30%的CCl4以诱导肝损伤,72 h后测定各组建鲤的生长指标、肝指数、生化指标、抗氧化指标、肝组织CYP450酶总活性及CYP1A和CYP3A的mRNA表达。结果表明:大黄素具有较强的清除DPPH自由基的能力,当大黄素浓度为6μg/mL时,其清除自由基的能力达81%;含3、5 g/kg大黄素的饲料能显著提高建鲤的相对增重率和特定生长率并显著降低饵料系数(P<0.05);含3、5 g/kg大黄素饲料能稳定肝细胞膜,显著抑制谷丙转氨酶(GPT)、谷草转氨酶(GOT)及乳酸脱氢酶(LDH)的溶出且显著恢复血清中白蛋白和总蛋白含量(P<0.05);大黄素可有效缓解建鲤氧化应激程度,能显著促进肝组织匀浆中总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)及谷胱甘肽过氧化物酶(GSH-Px)水平的恢复(P<0.05),同时显著抑制丙二醛(MDA)生成(P<0.05);大黄素能显著降低肝组织CYP450酶含量(P<0.05),抑制CCl4诱导的CYP1A、CYP3A基因表达上调。研究表明,饲料中添加3~5 g/kg大黄素具有显著的保肝作用,该作用与其抗击氧化、清除自由基、提高生长性能、抑制CCl4对CYP450酶的诱导作用有关。Juvenile common carp Cyprinus carpio Jian with initial body weight of about 150 g were reared in a circulating water system of 240 L tanks and fed basic diet(control group),and basic diets containing 30%CCl4(model control group),and emodin(EMD)at a dose of 1(low dose group),3(mid-dose group)and 5(high dose group)g/kg at water temperature of(27±2)℃with 2 parallel fish in each group and 10 parallel fish in each group for 60 days.Then the antioxidant activity of indices,hepato-somatic index,growth performances,biochemical indices,total activity of CYP450 in hepatopancreas were determined,and CYP1A and CYP3A mRNA expression level were detected in the common carp injected with 30%CCl4to induce liver injury intraperitoneally in model control group,and in emodin supplementation groups in order to fully evaluate the liver protection effects of emodin(EMD)on aquatic animals.The results showed that EMD had a strong scavenging ability of DPPH free radicals,with scavenging ability of 81%at 6 mg/mL dietary EMD.There were significantly increase in relative weight gain rate(RWR)and specific growth rate(SGR),and decrease in food conversion ratio(FCR)in the common carp in mid-dose group and high dose group.EMD led to stabilize the liver cell membrane,to inhibit the leakages of glutamate transaminase(GOT),glutamate transaminase(GPT),and lactate dehydrogenase(LDH),and to recovery levels of Alb and total protein(TP)in serum(P<0.05).The mitigation of oxidative stress,promotion of recovery of total antioxidant capacity(T-AOC),and activities of superoxide dismutase(SOD),glutathione(GSH)and glutathione peroxidase(GSH-Px)in liver homogenate,and significant inhibition of the synthesis of malondialdehyde(MDA)were observed in the common carp fed the diets containing EMD(P<0.05).There were significant decrease in the content of CYP450 enzyme in liver and inhibition of the up-regulation of mRNA expression of CYP1A and CYP3A induced by CCl4 in common carp fed the diets containing EMD.The findings indicated that diets containing 3-5 g/kg EMD

关 键 词：建鲤 四氯化碳 模型 CYP450 

分 类 号：Q948[生物学—植物学] S968.1[农业科学—水产养殖]