基于16S rRNA高通量测序的鳢肠道微生物群落结构研究  

Microbial community in intestine of snakehead based on 16S rRNA high-throughput sequencing

作　　者：徐晟云 陈昆慈[2] 罗青 刘海洋[2] 欧密 上官清 赵建[2] XU Shengyun;CHEN Kunci;LUO Qing;LIU Haiyang;OU Mi;SHANGGUAN Qing;ZHAO Jian(Key Laboratory of Tropical and Subtropical Aquatic Resources Utilization and Aquaculture, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China)

机构地区：[1]中国水产科学研究院珠江水产研究所,农业农村部热带亚热带水产资源利用与养殖重点实验室,广东广州510380 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《大连海洋大学学报》2020年第5期693-700,共8页Journal of Dalian Ocean University

基　　金：国家现代农业产业技术体系专项(CARS-46);中国水产科学研究院院级基本科研业务费(2020TD34);广东省现代农业产业技术体系创新团队建设项目(2019KJ150)。

摘　　要：为探究鳢肠道微生物与鳢健康养殖间的关系,采用Illumina HiSeq 2500高通量测序技术对不同养殖环境中成年健康乌斑杂交鳢Channa argus♀×Channa maculata♂(体质量100~150 g)及野生斑鳢(体质量50~100 g)肠道内容物进行微生物测序及信息分析。结果表明:不同环境(珠海斗门DM,佛山南海BRCT、BRSNC,佛山顺德杏坛XT,阳江YJ)鳢肠道内容物共31个样品间OTU数具有一定差别,总数达585,表明不同环境下鳢肠道内容物中优势菌群较为丰富;采集的样品中优势菌群主要为红球菌属Rhodococcus、梭菌属Clostridium、微杆菌Microbacterium、弧菌属Vibrio、气单胞菌属Aeromonas和邻单胞菌属Plesiomonas等,其中,梭菌属、红球菌属在所有样品中均存在,但不同环境所占的比例不同;热图分析显示,鳢肠道微生物群落中还包含变形杆菌属Proteus、肠球菌属Enterococcus、乳杆菌属Lactobacillus、拟杆菌属Bacteroides、棒杆菌属Corynebacterium等其他菌属,但占比不高,说明菌群数量不多;PCA分析显示,人工养殖杂交鳢(DM、BRCT、BRSIVC、XT)的肠道微生物群落结构比较接近,但与野生鳢(YJ)的群落结构具有一定差异。研究表明,相同环境下鳢肠道内容物中微生物群落菌属有所相似,不同环境条件下的鳢肠道微生物类群数量和优势菌群均存在显著差异。The microbes in intestinal contents were sequenced and analyzed in adult healthy hybrid snakehead Channa argus♀×C.maculate♂with body weight of 100-150 g collected from Doumen(DM)in Zhuhai,and a pond(BRCT)and cement pond(BRSNC)in Nanhai,and Shunde Xingtan(XT),and wild snakehead with body weitht of 50-100 g collected from Yangjian(YJ)in Foshan by Illumina hiseq 2500 to explore the relationship between the intestinal microorganism and the healthy breeding of snakehead.The results showed that there were some differences in the number of OTUs among the intestinal contents of snakehead in different environments,with the total number of 585,showing that the rich dominant microflora in the contents of the intestinal tract in different environments.Rhodococcus,Clostridium,Microbacterium,Vibrio,Aeromonas,Plesiomonas,Clostridium and Rhodococcus were the dominant flora in all samples,with different proportions in different environments.Heatmap analysis revealed that Proteus,Enterococcus,Lactobacillus,Lactobacillus,Bacteroides,and Corynebacterium were observed intestinal flora,with different proportion,indicating that the number of bacteria was not large.PCA analysis showed that there was the similar intestinal microbial community structure in the artificially-bred hybrid snakehead including DM,BRCT,BRSNC and XT,different from wild YJ.The findings indicated that there were the similar characteristics of microbial community in intestinal contents of snakehead in the same culture environment,and that the composition and diversity of intestinal microflora in different environments were quite different.

关 键 词：鳢 16S rRNA 肠道微生物 优势菌群 群落结构 

分 类 号：S964.3[农业科学—水产养殖]