水产动物精原干细胞和胚胎干细胞冷冻保存技术研究进展  

Research progress on cryopreservation of stem cells in spermatogonia and embryos in animals in aquaculture:a review

作　　者：赵谭军 刘丽[1] 常亚青[1] 湛垚垚[1] ZHAO Tanjun;LIU Li;CHANG Yaqing;ZHAN Yaoyao(Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China)

机构地区：[1]大连海洋大学农业农村部北方海水增养殖重点实验室,辽宁大连116023

出　　处：《大连海洋大学学报》2020年第5期748-754,共7页Journal of Dalian Ocean University

基　　金：国家重点研发计划项目(2018YFD0900105);全国农业科研杰出人才及其创新团队项目。

摘　　要：生殖细胞及胚胎的低温冷冻保存不仅是水产动物种质资源保存的重要技术手段之一,也是为水产动物优良新品种创制和培育提供基础育种材料的主要途径。精原干细胞和胚胎干细胞冷冻保存技术是近年来水产研究领域新兴的一种低温冷冻保存技术,与传统冷冻保存的精子或胚胎相比,冷冻保存的精原干细胞及胚胎干细胞具有自我更新及多向分化的潜能,同时还具有可以进行体外分化与移植等优势。本文综述了近年来水产动物精原干细胞和胚胎干细胞冷冻保存技术研究及其应用成果,以期进一步丰富和完善水产动物精子和胚胎冷冻及保存技术的资料体系,为充分利用低温冷冻保存技术提高水产经济动物优质种质资源保护和新种创制效率提供参考。Cryopreservation of germ cells or embryos has been considered as one of important technical approaches for aquatic animal germplasm resources preservation,and a main way to provide essential basic breeding materials for the creation and breeding of new aquatic animal varieties.In recent years,cryopreservation of spermatogonial stem cells(SSCs)or embryonic stem cells(ESs)has become a new cryopreservation technology in aquaculture research fields due to its many advantages including self-renewal potential,differentiation potential and in vitro transplantation compared to germ cells and embryos.We reviewed the recent research on SSCs and ESs cryopreservation techniques in animals in aquaculture and their applications in recent years in order to systematically understanding the application of cryopreservation technology of SSCs or ESs in aquaculture animal germplasm preservation and new species creation.The article here will further enrich and improve basic data of aquatic animal sperm and embryo cryopreservation and preservation techniques,and provide reference ith making full use of cryopreservation techniques to improve the protection of high-quality germplasm resources of economically important aquatic animals and the efficiency of new species breeding.

关 键 词：水产动物 精原干细胞 胚胎干细胞 冷冻保存 

分 类 号：S917.4[农业科学—水产科学]