盐度对南极冰藻Chlamydomonas sp.ICE-L细胞膜流动性及其脂肪酸脱氢酶基因表达的影响  

Effects of Salinity on Cell Membrane Fluidity and Fatty Acid Desaturases Gene Expression of Antarctic Ice Algae Chlamydomonas sp.ICE-L

作　　者：张欣[1] 綦晓青 缪锦来 郑洲 ZHANG Xin;QI Xiao-qing;MIAO Jin-lai;ZHENG Zhou(School of Basic Medicine, Qingdao University, Qingdao 266061, China;First Institute of Oceanography, MNR, Qingdao 266061, China;Laboratory for Marine Drugs and Bioproducts,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266061,China)

机构地区：[1]青岛大学基础医学院,山东青岛266061 [2]自然资源部第一海洋研究所,山东青岛266061 [3]青岛海洋科学与技术试点国家实验室,海洋药物与生物制品功能实验室,山东青岛266061

出　　处：《海岸工程》2020年第3期179-186,共8页Coastal Engineering

基　　金：中央级公益性科研院所基本科研业务费专项资金资助项目——南极冰藻(6-4)光修复酶及其修复DNA紫外辐射损伤分子机制研究(2016Q10);国家自然科学基金项目——极端骤变海冰环境中南极冰藻水通道蛋白的分子作用机制研究(41776203);山东省重点研发计划项目——共轭亚油酸异构体制备关键技术及其在脂质代谢紊乱医用食品中应用(2018YYSP024)。

摘　　要：深入研究了盐度对南极冰藻Chlamydomonas sp.ICE-L细胞膜流动性及其脂肪酸脱氢酶基因的调控。研究结果显示,在高盐条件下,南极冰藻细胞膜的流动性无显著变化;C20∶5,C20∶3,C18∶3是细胞膜中三种主要的不饱和脂肪酸,且随着盐度的升高,总不饱和脂肪酸的含量升高;实时荧光定量PCR结果表明,高盐胁迫下,脂肪酸脱氢酶基因Δ9CiFAD的表达量迅速升高,Δ12CiFAD和Δ6CiFAD表达调控有延迟现象;脂肪酸脱局酶基因ω3CiFAD1在高盐环境中表达量升高,而ω3CiFAD2在低盐环境中表达量升高,这与盐度条件下细胞膜脂肪酸组成的变化趋势基本一致。由此可知,在高盐环境下南极冰藻可以通过提高脂肪酸脱氢酶基因表达,增加膜脂不饱和脂肪酸含量,从而提高细胞膜的流动性,维持南极冰藻正常的生理功能。Regulation of salinity on cell membrane fluidity and fatty acid desaturases gene in Antarctic ice algae Chlamydomonas sp.ICE-L is studied.Under high salt conditions the fluidity of cell membrane in Antarctic ice algea Chlamydomonas.sp.ICE-L does not change significantly.Fatty acid analysis shows that C20∶5,C20∶3 and C18∶3 are the three main unsaturated fatty acids in the cell membrane and the content of total unsaturated fatty acids increase with the increase of salinity.The results of real-time PCR indicate that under high salt stress,the expression of fatty acid desaturases geneΔ9CiFAD increases rapidly and the regulation of the expressions ofΔ12CiFAD andΔ6CiFAD are delayed.The expression ofω3CiFAD1 increases in a high salt environment,while that ofω3CiFAD2 increases in a low salt environment.Such expressions of the fatty acid desaturases gene are consistent with the change trend of fatty acid compositions of cell membrane under salinity.It can be seen that under the high salt environment,Antarctic ice algae can increase the content of membrane lipid unsaturated fatty acids by increasing the expression of fatty acid desaturases gene,by which the fluidity of cell membrane is improved and the normal physiological functions of Antarctic ice algae can be maintained.

关 键 词：南极冰藻 膜的流动性 脂肪酸 脱氢酶基因 盐度 

分 类 号：Q949.2[生物学—植物学]