针刺不同穴位对慢性炎症痛模型大鼠血清IL-4及IL-10的影响  

Effects of Acupuncture at Different Acupoints on IL-4 and IL-10 in Rat Model with Chronic Inflammatory Pain

作　　者：郑宇 张露芬[2] 王春生 张帆[4] 杨磊 ZHENG Yu;ZHANG Lufen;WANG Chunsheng;ZHANG Fan;YANG Lei(Beijing Nuclear Industry Hospital,Beijing 100045,China;Beijing University of Chinese Medicine;The Second Outpatient,Aerospace Center Hospital;Beijing Hospital of TCM;Dongzhimen Hospital)

机构地区：[1]北京核工业医院,北京100045 [2]北京中医药大学 [3]航天中心医院第二门诊部 [4]北京中医医院 [5]东直门中医院

出　　处：《西部中医药》2020年第8期30-33,共4页Western Journal of Traditional Chinese Medicine

摘　　要：目的:观察针刺不同穴位对佐剂性关节炎模型大鼠血清中白细胞介素4(interleukin-4,IL-4)和白细胞介素10(interleukin-10,IL-10)水平的影响,并就其机制进行探讨。方法:将40只健康成年雄性Wistar大鼠随机分为正常对照组、佐剂模型组、佐剂关元组、佐剂针百会组、佐剂针足三里组,每组8只。除正常对照组外于每只大鼠右后足掌皮下注射佐剂0.1 mL造模,造模成功后佐剂针关元组、佐剂针百会组、佐剂针足三里组分别选取关元、百会、足三里穴进行针刺,进针后接韩式电针仪断续波,每次20 min,隔日治疗1次。观察大鼠体质量及一般状况,并于治疗后24、48、72 h分别观察局部足爪肿胀情况,同时于治疗72 h后测定血清IL-4、IL-10水平。结果:与正常对照组相比,佐剂模型组、佐剂针刺各组大鼠血清IL-4水平均呈升高趋势,其中以佐剂针百会组升高较为明显,差异有统计学意义(P<0.05);与佐剂模型组相比,佐剂针刺各组大鼠血清IL-4水平均呈升高趋势,其中佐剂针百会组显著升高,差异有统计学意义(P<0.05)。佐剂针刺各组中佐剂针关元组大鼠血清IL-4水平最低,佐剂针足三里组次之,而佐剂针百会组最高。与正常对照组相比,佐剂模型组和佐剂针关元组大鼠血清IL-10水平均降低,差异有统计学意义(P<0.01);佐剂针足三里和佐剂针百会组血清IL-10水平呈升高趋势,其中佐剂针足三里组与正常对照组相比,差异有统计学意义(P<0.05)。与佐剂模型组相比,佐剂针关元组大鼠血清IL-10水平降低,差异有统计学意义(P<0.05);佐剂针足三里和针百会组血清IL-10水平均明显升高,差异有统计学意义(P<0.01),其中佐剂针足三里组升高最为明显。佐剂各穴位组中佐剂关元组大鼠血清IL-10水平最低,佐剂百会组次之,而佐剂足三里组最高。结论:针灸佐剂性关节炎大鼠可以上调IL-4、IL-10水平,从而增强细胞因子的抗炎效应,但不同�Objective:To observe the effects of acupuncture at different acupoints on the levels of IL-4 and IL-10 in the serum of rat model with adjuvant arthritis,and discuss its mechanism.Methods:All 40 healthy male Wistar rats were randomized into normal control group,adjuvant model group,the group of adjuvant and acupuncture at Guanyuan(CV4,RN4),the group of adjuvant and acupuncture at Baihui(DU20)and the group of adjuvant and acupuncture at Zusanli(ST36),eight rats each group.Except normal control group,the rat models were established by subcutaneous injection of adjuvant,0.1mL,into the palm of the right hindfoot,after establishing the models successfully,the group of adjuvant and acupuncture at Guanyuan(CV4,RN4),the group of adjuvant and acupuncture at Baihui(DU20)and the group of adjuvant and acupuncture at Zusanli(ST36)chose needling at Guanyuan,Baihui and Zusanli respectively,after needling insertion,they were connected with intermittent wave of Korean electroacupuncture instrument,20 minutes each time,once every other day.To observe body mass and general situations of the rats,to observe local paw swelling after treating for 24,48 and 72 hours respectively,simultaneously to detect the levels of IL-4 and IL-1072 hours after treating.Results:Compared with normal control group,the levels of IL-4 in adjuvant model group and different groups of adjuvant needling were on the rise,among them,the increase of the group of adjuvant and acupuncture at Baihui was more notable,and the difference had statistical meaning(P<0.05);compared with adjuvant model group,the levels of IL-4 in different groups of adjuvant needling were on the rise,among them,the group of adjuvant and acupuncture at Baihui increased notably,and the difference had statistical meaning(P<0.05).Among these groups,the levels of IL-4 of the group of adjuvant and acupuncture at Guanyuan were the lowest,the group of adjuvant and acupuncture at Zusanli the next,while the group of adjuvant and acupuncture at Baihui the highest level.Compared with normal control gro

关 键 词：炎症痛 慢性 细胞因子 针刺 百会 足三里 关元 动物实验 

分 类 号：R245[医药卫生—针灸推拿学]