活血通脉汤对大鼠心肌缺血再灌注损伤的保护作用  

The Protective Effect of Decoction for Unblocking Channels and Promoting Blood Circulation on Myocardial Ischemia-Reperfusion Injury in Rats

作　　者：田兆华 TIAN Zhao-hua(Shenzhen Hospital of Beijing University of Traditional Chinese Medicine(Longgang),Shenzhen,Guangdong,China,518172)

机构地区：[1]北京中医药大学深圳医院(龙岗),广东深圳518172

出　　处：《河南中医》2020年第10期1531-1535,共5页Henan Traditional Chinese Medicine

摘　　要：目的:探讨活血通脉汤对大鼠心肌缺血再灌注损伤的保护作用。方法:将30只SD大鼠按照随机数字表法分为假手术组、模型组和活血通脉汤组,每组10只。假手术组与模型组均给予10 mL·kg^-1生理盐水灌胃,活血通脉汤组给予10 mL·kg^-1活血通脉汤水煎液灌胃,连续7 d。最后1次灌胃1 h后,假手术组在左冠状动脉前降支穿线但不结扎,模型组与活血通脉汤组在左冠状动脉前降支结扎30 min,再灌注90 min。检测各组大鼠血清超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)、肌酸激酶同工酶-MB(creatine kinase isoenzymes MB,CK-MB)、乳酸脱氢酶(lactate dehydrogenase,LDH)、心肌肌钙蛋白I(Cardiac troponin I,cTnI),检测大鼠缺血区心肌组织血管内皮生长因子(vascular endothelial growth factor,VEGF)、碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)、B淋巴细胞瘤-2基因(B-cell lymphoma-2,Bcl-2)、Bcl-2相关X蛋白(Bcl-2 Associated X Protein,BAX)、心肌细胞凋亡指数(apoptotic index,AI)。结果:活血通脉汤组SOD、GSH-PX、VEGF、bFGF、Bcl-2阳性率高于模型组,差异具有统计学意义(P<0.05);活血通脉汤组MDA、CK-MB、LDH、cTnI、Bax阳性率、AI低于模型组,SOD、GSH-PX低于假手术组,MDA、CK-MB、LDH、cTnI、VEGF、bFGF、Bcl-2阳性率、Bax阳性率、AI高于假手术组,差异均有统计学意义(P<0.05)。结论:活血通脉汤对大鼠心肌缺血再灌注损伤有明显保护作用,可以提高心肌抗氧化能力,减轻心肌细胞损伤程度,促进血管新生,改善心肌缺血。Objective:To probe into the protective effect of Decoction for Unblocking Channels and Promoting Blood Circulation on myocardial ischemia-reperfusion injury in rats.Methods:A total of 30 SD rats were divided into the sham operation group,the model group and the group of Decoction for Unblocking Channels and Promoting Blood Circulation according to random number table method,with 10 rats in each group.Both the sham operation group and the model group were given 10 mL·kg^-1 normal saline by gavage,while the group of Decoction for Unblocking Channels and Promoting Blood Circulation was given 10 mL·kg^-1 of Decoction for Unblocking Channels and Promoting Blood Circulation by gavage for 7 days.One hour after the last gavage,the left anterior descending coronary artery was threaded but not ligated in the sham operation group,while in the model group and the group of Decoction for Unblocking Channels and Promoting Blood Circulation,the left anterior descending coronary artery was ligated for 30 minutes,and then reperfused for 90 minutes.In each group,serum superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-PX),creatine kinase isozymes MB(CK-MB),lactate dehydrogenase(LDH),cardiac troponin I(cTnI),vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF),B-cell lymphama-2(Bcl-2) and Bcl-2 associated X protein(Bcl-2) Protein(BAX),and apoptotic index(AI) were detected respectively.Results:The positive rates of SOD,GSH-PX,VEGF,bFGF and Bcl-2 in the group of Decoction for Unblocking Channels and Promoting Blood Circulation were higher than those in the model group,and all the differences were statistically significant(P<0.05).The positive rates of MDA,CK-MB,LDH,cTnI,Bax and AI in the group of Decoction for Unblocking Channels and Promoting Blood Circulation were lower than those in the model group,SOD and GSH-Px were lower than those in the sham operation group;the positive rates of MDA,CK-MB,LDH,cTnI,VEGF,bFGF,Bcl-2,BAX and AI in the group of Decoction for Unblocking Channels and

关 键 词：心肌缺血再灌注损伤 活血通脉汤 抗氧化 血管新生 心肌细胞凋亡 大鼠 

分 类 号：R285.5[医药卫生—中药学]