抗酸染色与荧光定量PCR检测在淋巴结结核诊断中的应用  

Application of Acid-fast Staining and Fluorescence Quantitative PCR in the Diagnosis of Lymph Node Tuberculosis

作　　者：黄蓉飞[1] 蔡玮 樊元春[1] 王晓燕[1] Huang Rongfei;Cai Wei;Fan Yuanchun;Wang Xiaoyan(Department of Pathology, The First Affiliated Hospital of Chengdu Medical College, Chengdu 610500, China;Department of Pathology, Mingshan District People’s Hospital of Ya’an City, Ya’an 625100, China)

机构地区：[1]成都医学院第一附属医院病理科,成都610500 [2]雅安市名山区人民医院病理科,雅安625100

出　　处：《成都医学院学报》2020年第5期630-633,共4页Journal of Chengdu Medical College

摘　　要：目的探讨提高淋巴结空芯针穿刺活检(CNB)诊断淋巴结结核的准确性。方法选取2016年1月1日至2019年12月31日在成都医学院第一附属医院病理科收集的淋巴结CNB病例共68例,进行抗酸染色与荧光定量PCR检测,并将两项辅助检测联合使用与单独使用情况对淋巴结结核的诊断效果进行比较。结果联合使用抗酸染色与荧光定量PCR检测结核的敏感性、特异性和确诊率分别为65.62%(42/64)、100.00%(4/4)、61.76%(42/68),高于单独使用一项辅助检测的确诊率。对于不伴有坏死的肉芽肿性炎病例,辅助检测阴性的比率较高。结论联合应用抗酸染色和PCR检测能提高淋巴结CNB结核诊断的准确率,具有临床应用价值。Objective To improve the accuracy of core needle biopsy(CNB)in the diagnosis of lymph node tuberculosis.Methods A total of 68 lymph node CNB cases collected in the pathology department of the First Affiliated Hospital of Chengdu Medical College from January 1,2016 to December 31,2019 were selected for acid-fast staining and fluorescence quantitative PCR detection.And the diagnosis effect of combined use of the two auxiliary tests on lymph node tuberculosis was compared with that of single use.Results The sensitivity,specificity and diagnosis rates of tuberculosis detected by acid-fast staining combined with fluorescence quantitative PCR were 65.62%(42/64),100%(4/4)and 61.76%(42/68),respectively,which were higher than the diagnosis rates of only one auxiliary test.For granulomatous inflammation without necrosis,the negative rate of auxiliary test was higher.Conclusion The combination of acid-fast staining and fluorescence quantitative PCR detection can improve the diagnostic accuracy of CNB lymph node tuberculosis,which is of clinical application value.

关 键 词：空芯针穿刺活检 淋巴结结核 PCR 诊断 联合应用 

分 类 号：R521.2[医药卫生—内科学]