三种铁源对珍珠龙胆石斑鱼幼鱼生长性能、肝脏抗氧化酶活性及肠道发育形态的影响  

Effects of Three Kinds Fe Sources on Growth Performance,Hepatic Antioxidative Enzymes and Intestinal Morphology of Juvenile Pearl Gentian Grouper(Epinephelus lanceolatus♂×Epinephelus fuscoguttatus♀)

作　　者：郭鑫伟 张洋 迟淑艳[1,3] 谭北平 姚亚军[4] 姚红梅 GUO Xin-Wei;ZHANG Yang;CHI Shu-Yan;TAN Bei-Ping;YAO Ya-Jun;YAO Hong-Mei(Laboratory of Aquatic Animal Nutrition and Feed, Guangdong Ocean University, Zhanjiang 524088, China;Research Center for Accurate Nutrition and Highly Efficient Feed Engineering of Aquatic Animals of Guangdong Province, Zhanjiang 524088, China;Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China of Ministry of Agriculture, Zhanjiang 524088, China;Changsha Xingjia Biotechnology Share Co., Ltd, Cahngsha 410128, China)

机构地区：[1]广东海洋大学水产动物营养与饲料实验室,广东湛江524088 [2]广东省水产动物精准营养与高效饲料工程技术研究中心,广东湛江524088 [3]农业农村部南方水产与畜禽饲料重点实验室,广东湛江524088 [4]长沙兴嘉生物工程股份有限公司,湖南长沙410128

出　　处：《中国海洋大学学报（自然科学版）》2020年第11期53-61,共9页Periodical of Ocean University of China

基　　金：广东省科技计划项目公益研究与能力建设专项项目(2016A020206006);现代农业产业技术体系专项资金项目(CARS-47)资助。

摘　　要：为研究配合饲料中添加不同铁源对珍珠龙胆石斑鱼(Epinephelus lanceolatus♂×Epinephelus fuscoguttatus♀)幼鱼的影响。选取遗传背景一致的健康珍珠龙胆石斑鱼(初重(9.00±0.49)g)270尾,随机分成3个处理,分别饲喂硫酸亚铁(FeSO4)、甘氨酸亚铁(Fe-Gly(II))和羟基蛋氨酸铁(Fe-MHA)3种铁源等氮等脂的试验饲料,养殖8周,测量各组珍珠龙胆石斑鱼的生长性能、抗氧化能力和肠道发育等形态结构指标。研究表明:(1)铁源对各组间成活率、增重率和特定生长率均无显著影响(P>0.05),但Fe-MHA组肥满度、全鱼和脊椎骨中Fe含量显著高于其余2组(P<0.05)。(2)Fe-MHA组肝脏CAT活性显著高于Fe-Gly(II)组和FeSO4组(P<0.05),且该组肝脏MDA含量显著低于Fe-Gly(II)组和FeSO4组(P<0.05)。(3)摄食Fe-MHA组饲料的石斑鱼前、中、后肠的绒毛高度显著高于FeSO4组和Fe-Gly(II)组(P<0.05),而绒毛宽度显著低于FeSO4组和Fe-Gly(II)组(P<0.05);该组中肠段肌层厚度显著高于Fe-Gly(II)组和FeSO4组(P<0.05)。研究结果表明,与Fe-Gly(II)和FeSO4相比,Fe-MHA有利于机体对铁元素的沉积,改善鱼体肠道发育,显著提高珍珠龙胆石斑鱼幼鱼肝脏的抗氧化能力,建议珍珠龙胆石斑鱼幼鱼饲料中添加的铁源形式为Fe-MHA。The three kinds of iron sources iron sulfate(FeSO4),glycine chelated iron(II)(Fe-Gly(II)),and iron hydroxymethionine(Fe-MHA)were added in the juvenile pearl gentian grouper(Epinephelus lanceolatus♂×E.fuscoguttatus♀)isonitrogenous and isolipidic experimental diets respectively.The grouper(initial weight(9.00±0.49)g)were randomly divided into 3 groups with 3 replicates,and 30 individuals in each replicate.The experiment lasted 8 weeks.The effects of three different iron sources on the growth performance,hepatic antioxidative abilities,and intestinal morphology of were evaluated.The results showed that although three kinds of Fe sources did not influenced significantly the survival ratio,weight gain ratio,and specific growth rate(P>0.05),the condition factor,Fe contentsof whole body and vertebrae of fish fed Fe-MHA diet were significantly higher than those of fish fed FeSO4and Fe-Gly(II)diets(P<0.05).Hepatic CAT activities in Fe-MHA group was significantly higher than that in other two groups(P<0.05).MDA contents of liver in Fe-MHA group was significantly lower than that in other two groups(P<0.05).The plica height of the three part of the fish intestinal in Fe-MHA group were significantly higher than those in FeSO4 and Fe-Gly(II)groups(P<0.05),the plica width of foregut,midgut and hindgut in Fe-MHA group were significantly lower than that in other two groups(P<0.05).The muscle thickness of midgut in Fe-MHA group was significantly higher than that in FeSO4 and Fe-Gly(II)groups(P<0.05).Based on the above experimental results,compared with Fe-Gly(II)and FeSO4,adding Fe-MHA in grouper diet improved the iron deposition and intestinal development,and then ameliorate antioxidant capacity of juvenile pearl gentian grouper.It was recommended that Fe-MHA should be better as the iron sources in the feed of juvenile pearl gentian grouper.

关 键 词：铁源 珍珠龙胆石斑鱼 生长性能 抗氧化酶 矿物元素沉积 肠道发育形态 

分 类 号：S917.4[农业科学—水产科学]