PRRSV不同毒株RT-PCR检测方法的建立与应用  

Establishment and application of RT-PCR methods for identifying different types of PRRSV

作　　者：饶云萍 Rao Yunping(Jofunhwa Biotechnology(Fuzhou)Co.Ltd,Fuzhou350014)

机构地区：[1]兆丰华生物科技(福州)有限公司,福州350014

出　　处：《福建畜牧兽医》2020年第5期1-3,共3页Fujian Journal of Animal Husbandry and Veterinary medicine

摘　　要：根据GenBank中猪繁殖与呼吸综合征病毒经典毒株、高致病性毒株与流行株NADC30-like Nsp2的特点设计特异性引物,建立能够快速区分这三类毒株的RT-PCR检测方法。该方法能够对猪繁殖与呼吸综合征病毒的经典毒株、高致病性毒株与NADC30-like流行株扩增出特异性片段,其大小分别为1000 bp、900 bp和700 bp。具有快速、特异、通用等特点,可用于实验室快速诊断,为该病的诊断及免疫防控提供参考依据。用本试验所建立的方法对福建省疑似猪繁殖与呼吸综合征病毒感染的58份样品进行检测,结果显示34份为猪繁殖与呼吸综合征病毒阳性,其中高致病性毒株占41.18%,NADC30-like占14.71%,多毒株共感染占35.29%,提示高致病性毒株仍占主导,但多毒株共感染成为流行趋势。Based on the characteristics of porcine reproductive and respiratory syndrome virus(PRRSV)gene in Nsp2 between Classical PRRSV,HP-PRRSV and NADC30-like,one pair of primers was designed and a rapid diagnostic method of RT-PCR was established.The established method had a rapid and general features and it only amplified target band from PRRSV.A total of 1000 bp bands were amplified from Classical PRRSV and 900 bp bands were amplified from HP-PRRSV strains and 700 bp bands were amplified from NADC30-like strains.A highly special RT-PCR method was established and it can directly differentiate PRRSV,which can be provide reference for diagnosis and the immune prevention.Using the RT-PCR method established in this experiment,58 samples of suspected PRRSV infections collected from many areas in Fujian were tested.The 34 PRRSV strains were detected,The detection rate of HP-PRRSV was 41.18%,NADC30-like was 14.71%,mixed infection was 35.29%.The result suggested the HP-PRRSV strains still dominate,but mixed infection with multiple strains has become an epidemic.

关 键 词：猪繁殖与呼吸综合征 经典毒株 高致病性毒株 NADC30-like 

分 类 号：S852.651[农业科学—基础兽医学]