基于氧化碳纳米颗粒为受体的荧光共振能量转移体系的构建及汞离子检测应用  

Construction of Fluorescence Resonance Energy Transfer System UsingOxidized Carbon Nanoparticles as Acceptors and the Application ofMercury Ion Detection

作　　者：何梦媛 尚宁 朱倩茹 闫露露 刘畅 HE Mengyuan;SHANG Ning;ZHU Qianru;YAN Lulu;LIU Chang(College of Chemistry and Chemical Engineering,Xinyang Normal University,Xinyang 464000,China)

机构地区：[1]信阳师范学院化学化工学院,河南信阳464000

出　　处：《信阳师范学院学报（自然科学版）》2020年第4期630-634,共5页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(21804117);河南省科技攻关项目(182102310703);河南省高等学校重点科研项目(18B150022);信阳师范学院“南湖学者奖励计划”青年项目。

摘　　要：通过超声辅助硝酸氧化法制备了尺寸均一、分散性好、猝灭性能优良的氧化碳纳米颗粒(OCNPs).构建了以荧光染料FAM为供体,以OCNPs为受体,以富含T碱基的ssDNA作为识别分子的荧光共振能量转移(FRET)体系.OCNPs与单链核酸作用,拉近供受体之间的距离,供体的荧光猝灭,当存在Hg 2+时,Hg 2+与T碱基形成T-Hg 2+-T结构,供受体之间距离增大,供体的荧光恢复.基于此建立了“off-to-on”型Hg 2+荧光传感器,检测线性范围为0.1~10.0 nmol/L,检出限为0.06 nmol/L.此方法对Hg 2+具有良好的选择性,将其应用于自来水和河水中Hg 2+的检测,加标回收率为97.4%~108.4%.Oxidized carbon nanoparticles(OCNPs)with uniform size,good dispersion and excellent quenching performance were prepared by ultrasonic assisted nitric acid oxidation method.A fluorescence resonance energy transfer(FRET)system was constructed,with FAM as energy donors,OCNPs as energy acceptors and T-rich ssDNA as recognition molecules.The interaction between OCNPs and ssDNA brought the energy donor and acceptor in close proximity,leading to the quenching of fluorescence of FAM.After the addition of mercury ion(Hg 2+),the distance between the donor and acceptor was increased and the fluorescence of FAM was recovered gradually,due to the formation of T-Hg 2+-T structure.Based on the above-mentioned principle,an“off-to-on”fluorescence sensor was established for Hg 2+detection.The linear range of mercury ion detection was 0.1~10.0 nmol/L,and the detection limit was 0.06 nmol/L.The method showed good sensitivity and has been applied to the determination of Hg 2+in tap water and river water with a recovery of 97.4%~108.4%.

关 键 词：氧化碳纳米颗粒 荧光共振能量转移 Hg^2+检测 

分 类 号：O657.39[理学—分析化学]