不同温度下形成的深海菌膜对厚壳贻贝幼虫变态的影响  

Effects of biofilms of deep-sea bacteria at varying temperatures on larval metamorphosis of Mytilus coruscus

作　　者：梁箫[1,2,3] 杨丽婷 彭莉华 魏玉利[1,4] 方家松 杨金龙[1,2,3] LIANG Xiao;YANG Liting;PENG Lihua;WEI Yuli;FANG Jiasong;YANG Jinlong(International Research Center for Marine Biosciences,Ministry of Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory,Guangzhou 511458,China;Hadal Science and Technology Research Center,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学,国家海洋生物科学国际联合研究中心,上海201306 [2]上海海洋大学,水产种质资源发掘与利用教育部重点实验室,上海201306 [3]南方海洋科学与工程广东省实验室,广东广州511458 [4]上海海洋大学,深渊科学技术中心,上海201306

出　　处：《水产学报》2020年第10期1728-1741,共14页Journal of Fisheries of China

基　　金：国家自然科学基金(41876159,41476131);南方海洋科学与工程广东省实验室人才团队引进重大专项(GML 2019ZD0402);国家重点研发计划(2018YFC0310600)。

摘　　要：为探讨深海细菌生物被膜对温度的适应性及对厚壳贻贝幼虫变态的影响,分别在4、18、25、37℃条件下培养生物被膜,调查温度对细菌密度、膜厚和胞外产物等生物学特性的影响,以及被膜对幼虫变态发育的影响。结果显示,4个温度条件下产生的生物被膜均可有效促进幼虫变态发育。其中,深海菌株Virgibacillus sp.1在18℃时生物被膜的变态诱导活性最高(35%),且α胞外多糖含量较高,诱导活性与温度和细菌密度均显著相关;同时温度与其细菌密度和膜厚均显著相关。2株深海假交替单胞菌诱导活性与细菌密度均显著相关,温度仅影响Pseudoalteromonas sp.33细菌密度。研究表明,3株深海细菌均有很好的温度适应性,且都能形成生物被膜;温度变化导致生物被膜的生物学特性改变,最终影响其对幼虫的诱导效果。Effects of water temperature(4,18,25 and 37°C)on deep-sea bacterial biofilms and subsequent effect on metamorphosis of larvae of the mussel Mytilus coruscus were studied.The confocal laser scanning microscopy technique was used to clarify the characteristics of biofilms including biofilm thickness and the distribution of extracellular polymeric substances.The biofilms formed by the deep-sea bacteria could promote the metamorphosis of larvae under four temperature conditions.Virgibacillus sp.1 showed the highest inductive activity(35%),with highα-extracellular polysaccharide secretion.The Virgibacillus sp.1 biofilm inducing activities on larval metamorphosis were correlated to seawater temperature and bacterial density.Temperature was significantly correlated with bacterial density,induction activity and biofilm thickness.The densities of bacteria in the biofilms of the two Pseudoalteromonas strains were significantly correlated with the induced activity.The present study shows that deep-sea bacteria exhibited good potential for temperature change to form the biofilms,and impacted larval metamorphosis.

关 键 词：厚壳贻贝 深海细菌 幼虫变态 生物被膜 温度 

分 类 号：Q958[生物学—动物学] S917.1[农业科学—水产科学]