紫外分光光度法测定木豆叶中芪类化合物的含量  

Deterination of Stilbene in Cajanus cajan(L.)Millsp.by UV Spectrophotometry

作　　者：周伊宁 刘宇航 刘卫东 于海泉 Zhou Yining;Liu Yuhang;Liu Weidong;Yu Haiquan(Inner Mongolia Medical University,Huhhot 010010,China;Inner Monolian grand pharmaceutical Co.Ltd,Huhhot 010010,China;Inner Mongolia Enterprise Key Laboratory of Innovative Mongolian Medicine Research,Huhhot 010010,China)

机构地区：[1]内蒙古医科大学,呼和浩特010100 [2]大唐药业股份公司,呼和浩特010010 [3]内蒙古自治区蒙药新药研发企业重点实验室,呼和浩特010010

出　　处：《北方药学》2020年第9期9-11,共3页Journal of North Pharmacy

基　　金：内蒙古重大专项:蒙医药现代化关键技术研究及蒙药产业化-黄芪、甘草、蓝刺头三味地道蒙药材规范化种植及相关蒙药新药“甘蓝强骨片”临床前研究。

摘　　要：目的:建立木豆叶中芪类化合物的含量测定方法。方法:采用紫外分光光度法在320nm处,以白藜芦醇为对照品测定吸收度,并计算其含量。结果:芪类化合物测定浓度的线性范围为1.300~7.150μg/ml(r 2=0.9960);平均回收率为101.54%,RSD=0.73%(n=6)。结论:该法简便,重复性、稳定性良好,可作为木豆叶中芪类化合物含量测定方法。Objective:To set up a method for the determination of stilbene in Cajanus cajan(L.)Millsp.extracts.Method:The determination was performed at a wavelength of 320 nm.The absorbability was detected with resveratrol as control and its content was computed.Result:The linear range of stilbene was 1.300~7.150μg/ml(r 2=0.9960)and its average recovery was 101.54%,RSD=0.73%(n=6).Conclusion:The method is simple and sensitive with good stability,it can be suitable for the quality control of stilbene in Cajanus cajan(L.)Millsp.

关 键 词：木豆叶 芪类化合物 分光光度法 含量测定 

分 类 号：R284.1[医药卫生—中药学]