基于SSR分子标记的红安县省沽油遗传多样性分析  

Genetic Diversity of Staphylea bumalda DC in Hong’an County Based on SSR Markers

作　　者：李静[1] 田思雨 毛威涛 姚国新[1,2] LI Jing;TIAN Siyu;MAO Weitao;YAO Guoxin(School of Life and Science Technology,Hubei Engineering University,Xiaogan 432000,China;Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement,Wuhan 430000,China)

机构地区：[1]湖北工程学院生命科学技术学院,湖北孝感432000 [2]粮食作物种质创新与遗传改良湖北省重点实验室,湖北武汉430000

出　　处：《河南农业科学》2020年第10期42-47,共6页Journal of Henan Agricultural Sciences

基　　金：粮食作物种质创新与遗传改良湖北省重点实验室开放课题项目(20191zjj09);湖北省教育厅科学技术研究项目(B2018163)。

摘　　要：为加强湖北省红安县省沽油(Staphylea bumalda DC.)野生资源保护利用,了解红安县省沽油的遗传多样性概况,在红安县境内24个点采集24份省沽油野生种质资源,利用有多态性的21对SSR引物对红安县省沽油样本进行遗传多样性分析,并进行聚类分析。结果表明,21对SSR引物共检测到56个等位变异,平均每对引物可检测2.95个等位变异;有效等位基因数(Ne)为0.6354~2.7482个,平均为1.6223个;Shannon-Weaver多样性指数(I)为0.2963~1.7319,平均为0.7729;Nei’s遗传多样性指数为0.1574~0.7625,平均为0.3618;多态性信息含量(PIC)为0.1154~0.7523,平均为0.4389,所筛选的21对引物能较好地反映红安县省沽油不同地点间的遗传多样性信息。聚类分析结果表明,遗传相似系数(GS)为0.58~0.93,平均值为0.78,可将红安县省沽油分为两大类。In order to strengthen protection,utilization and understand genetic diversity of wild germplasm of Staphylea bumalda DC in Hong’an county,24 wild germplasms of Staphylea bumalda DC were collected in different sites.Genetic diversity was studied using 21 pairs of polymorphic SSR primers,and cluster analysis was done.The results showed that a total of 56 allelic variations were detected by 21 pairs of SSR primers,and 2.95 allelic variations were detected for each pair of primers;effective alleles number(Ne)ranged from 0.6354 to 2.7482,and the average number was 1.6223;Shannon-Weaver diversity index(I)ranged from 0.2963 to 1.7319,and the average was 0.7729;Nei’s genetic diversity index(H)ranged from 0.1574 to 0.7625,and the average was 0.3618;polymorphism information content(PIC)ranged from 0.1154 to 0.7523,and the average was 0.4389.The 21 pairs of SSR primers could better reflect the genetic diversity information of Staphylea bumalda DC from different sites.The cluster results showed that the genetic similarity coefficient(GS)ranged from 0.58 to 0.93,and the average was 0.78;Staphylea bumalda DC were divided into two broad categories.

关 键 词：省沽油 SSR标记 遗传多样性 聚类分析 红安县 

分 类 号：S793.9[农业科学—林木遗传育种]