屠宰场土壤蛋白分解菌的分离与鉴定  

Isolation and identification of soil protein decomposing bacteria in slaughterhouse

作　　者：王致远 陶志东 李富贵 马海锐 马娜娜 蔡新东 冉权 丁功涛 Wang Zhiyuan;Tao Zhidong;Li Fugui;Ma Hairui;Ma Na'na;Cai Xindong;Ran Quan;Ding Gongtao(Life Science and Engineering College,Northwest Minzu University,Gansu Lanzhou 730124;Biomedical Research Center,Northwest Minzu University,Gansu Lanzhou 730030)

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730124 [2]西北民族大学生物医学研究中心,甘肃兰州730030

出　　处：《现代畜牧兽医》2020年第10期9-13,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：“科技助力经济2020”重点专项“屠宰牛血液废弃物的生物处理与资源化利用(SQ2020YFF0405583)”;西北民族大学2019年国家级大学生创新创业训练计划项目“血红蛋白分解菌的筛选与发酵条件的优化(201910742099)”;中央高校基本科研业务经费“屠宰血液蛋白废弃物的资源化利用(31920200115)”;教育部“创新团队发展计划(IRT_17R88)”。

摘　　要：为减少屠宰场废弃蛋白造成的环境污染,同时为利用废弃蛋白制备氨基酸液体肥获取必要的菌株,试验以兰州市牛羊屠宰场污水池的土样为材料,分离筛选高效的蛋白分解菌。试验筛选得到一株目标菌株,经过形态学鉴定、生理生化和分子生物学鉴定后,该菌株为费格森埃希菌Ehrlich bacteria sp.,编号为NwMCC01910027,蛋白酶活力为69.52 U/mL.In order to reduce the environmental pollution caused by the waste protein in the slaughterhouse and obtain the necessary strains for utilization of waste protein in the preparation of amino acid liquid fertilizer,the experiment used the soil samples from the sewage pond of the cattle and sheep slaughterhouse in Lanzhou as the material to separate and screen for efficient protein decomposition bacteria.A target strain was screened in the experiment.After morphological identification,physiological,biochemical and molecular identification,the strain was Ehrlich bacteria sp.Ferguson,numbered NwMCC01910027,and the protease activity was 69.52 U/mL.

关 键 词：蛋白分解 菌株 分离 鉴定 酶活性 

分 类 号：S811.6[农业科学—畜牧学]